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Biophysics - Lecture

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Data

Official data in SubjectManager for the following academic year: 2020-2021

Course director

Dr. Beáta BUGYI (beata.bugyi@aok.pte.hu), associate professor

Department of Biophysics

Subject data

Code of subject: OBA-106-E  |  2 credit  |  Biotechnology |  Basic module  |  autumn

Prerequisites: -

Exam course: yes

Number of hours/semester

28 lectures + 0 practices + 0 seminars = total of 28 hours

Course headcount limitations

min. 1

Topic

The aim of the Biophysics curse is to introduce the students to the methods and applications, which are routinely used in both the medical and pharmaceutical biotechnology. The principles of the state-of the art approaches and instrumentations are covered by the topics. The course presents diverse spectroscopic techniques (absorption, fluorescence, infrared, Raman, EPR, NMR) imaging approaches (light and fluorescence microscopy, EM, super-resolution fluorescence microscopy, MRI), radioactive applications and calorimetric and fast kinetics techniques. The lectures discuss in details the physical basis and principles of each approach and the field of applications. The practicals are dedicated to extend the students’ knowledge and routine in the use of the different techniques. The practicals lay special emphasis on presenting not only the routine applications, but advanced uses of each technique. The limitations, as well as the artefacts that can be caused by improper experimental planning are highlighted.

Lectures

  • 1. Introduction - Dr. Bugyi Beáta
  • 2. Databases, resources. - Dr. Bugyi Beáta
  • 3. Electromagnetic radiations. - Dr. Grama László
  • 4. Quantummechanical model of atoms, quantum numbers. - Dr. Grama László
  • 5. Energy levels of atoms and molecules. - Dr. Lukács András Szilárd
  • 6. Laser. - Dr. Lukács András Szilárd
  • 7. UV-VIS spectroscopy. Circular dichroism (CD) spectroscopy. - Dr. Huber Tamás
  • 8. Infrared and Raman spectroscopy. - Dr. Bugyi Beáta
  • 9. Fluorescence spectroscopy, fluorescence parameters. Förster resonance energy transfer (FRET). - Huberné Barkó Szilvia
  • 10. Flow cytometry. Fluorescence activated cell sorting. - Huberné Barkó Szilvia
  • 11. Electron paramagnetic resonance (EPR), nuclear magnetic resonance (NMR) spectroscopy. - Dr. Grama László
  • 12. Magnetic resonance imaging (MRI). - Dr. Grama László
  • 13. Basics of light microscopy and fluorescence microscopy. - Dr. Bugyi Beáta
  • 14. Advanced fluorescence microscopy: confocal microscopy, fluorescence recovery after photobleaching (FRAP), multi-photon microscopy, FRET, FLIM, TIRFM, STED, SIM, single molecule localization microscopy. - Dr. Bugyi Beáta
  • 15. Scanning probe microscopy, electron microscopy. - Dr. Bugyi Beáta
  • 16. Image analysis. - Dr. Bugyi Beáta
  • 17. X-ray diffraction. - Dr. Talián Csaba Gábor
  • 18. Rapid kinetic methods: stopped-flow, surface plasmon resonance (SPR). - Dr. Kengyel András Miklós
  • 19. Sound. Ultrasound. - Dr. Kengyel András Miklós
  • 20. Radioactivity, interaction of radioactive radiations with matter. - Dr. Szabó-Meleg Edina
  • 21. Dosimetry, detection of radioctive radiations. - Dr. Szabó-Meleg Edina
  • 22. Biological effects of radioactive radiations. - Dr. Kengyel András Miklós
  • 23. Gamma-camera, computed tomography (CT), single-photon emission computed tomography (SPECT), positron emission tomography (PET). - Dr. Kengyel András Miklós
  • 24. Thermodynamics: laws, thermodynamic potentials. - Dr. Lukács András Szilárd
  • 25. Calorimetric methods: differential scanning calorimetry (DSC), isothermal titration calorimetry (ITC). - Dr. Lukács András Szilárd
  • 26. Analytical separation techniqes: sedimentation, electrophoresis, chromatography. - Dr. Talián Csaba Gábor
  • 27. Mass spectrometry. - Dr. Talián Csaba Gábor
  • 28. Revision - Dr. Bugyi Beáta

Practices

Seminars

Reading material

Obligatory literature

Literature developed by the Department

http://www.biofizika.aok.pte.hu/tantargy/Molecular_Biophysics_Lectures_-_Biotechnology

Notes

http://www.biofizika.aok.pte.hu/tantargy/Molecular_Biophysics_Lectures_-_Biotechnology

Recommended literature

Conditions for acceptance of the semester

Maximum of 15 % absence allowed

Mid-term exams

A kurzus oktatóival egyeztetve.

Making up for missed classes

A kurzus oktatóival egyeztetve.

Exam topics/questions

http://www.biofizika.aok.pte.hu/tantargy/Molecular_Biophysics_Lectures_-_Biotechnology

Examiners

  • Dr. Bugyi Beáta
  • Dr. Bukovics Péter

Instructor / tutor of practices and seminars

  • Dr. Bugyi Beáta