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Biophysics - Practice

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Data

Official data in SubjectManager for the following academic year: 2020-2021

Course director

Dr. Beáta BUGYI (beata.bugyi@aok.pte.hu), associate professor

Department of Biophysics

Subject data

Code of subject: OBA-106-G  |  1 credit  |  Biotechnology |  Basic module  |  autumn

Prerequisites: OBA-106-E parallel

Number of hours/semester

0 lectures + 14 practices + 0 seminars = total of 14 hours

Course headcount limitations

min. 1

Topic

The aim of the Biophysics curse is to introduce the students to the methods and applications, which are routinely used in both the medical and pharmaceutical biotechnology. The principles of the state-of the art approaches and instrumentations are covered by the topics. The course presents diverse spectroscopic techniques (absorption, fluorescence, infrared, Raman, EPR, NMR) imaging approaches (light and fluorescence microscopy, EM, super-resolution fluorescence microscopy, MRI), radioactive applications and calorimetric and fast kinetics techniques. The lectures discuss in details the physical basis and principles of each approach and the field of applications. The practicals are dedicated to extend the students’ knowledge and routine in the use of the different techniques. The practicals lay special emphasis on presenting not only the routine applications, but advanced uses of each technique. The limitations, as well as the artefacts that can be caused by improper experimental planning are highlighted.

Lectures

Practices

  • 1. Introduction
  • 2. Data bases, resources
  • 3. Laser
  • 4. Raman and infrared spectroscopy
  • 5. UV-VIS spectroscopy
  • 6. Fluorescence spectroscopy 1: steady state spectroscopy
  • 7. Fluorescence spectroscopy 2: time dependent spectroscopy
  • 8. Light microscopy
  • 9. Image analysis
  • 10. Rapid kinetic measurements: stopped-flow
  • 11. Ultrasound
  • 12. Revision
  • 13. Preparation for the test/consultation
  • 14. Test

Seminars

Reading material

Obligatory literature

Literature developed by the Department

http://www.biofizika.aok.pte.hu/tantargy/Molecular_Biophysics_Practicals_-_Biotechnology

Notes

http://www.biofizika.aok.pte.hu/tantargy/Molecular_Biophysics_Practicals_-_Biotechnology

Recommended literature

Conditions for acceptance of the semester

Maximum of 15 % absence allowed

Mid-term exams

A written test during the semester.

Making up for missed classes

Can be discussed with the course leader.

Exam topics/questions

http://www.biofizika.aok.pte.hu/tantargy/Molecular_Biophysics_Practicals_-_Biotechnology

Examiners

Instructor / tutor of practices and seminars

  • Dr. Bugyi Beáta
  • Dr. Bukovics Péter
  • Dr. Huber Tamás
  • Dr. Kengyel András Miklós
  • Dr. Szabó-Meleg Edina
  • Karádi Kristóf
  • Pirisi Katalin Erzsébet