Data
Official data in SubjectManager for the following academic year: 2020-2021
Course director
-
Dr. Beáta BUGYI
associate professor,
Department of Biophysics -
Number of hours/semester
lectures: 0 hours
practices: 14 hours
seminars: 0 hours
total of: 14 hours
Subject data
- Code of subject: OBA-106-G
- 1 kredit
- Biotechnology MSc
- Basic modul
- autumn
OBA-106-E parallel
Course headcount limitations
min. 1
Topic
The aim of the Biophysics curse is to introduce the students to the methods and applications, which are routinely used in both the medical and pharmaceutical biotechnology. The principles of the state-of the art approaches and instrumentations are covered by the topics. The course presents diverse spectroscopic techniques (absorption, fluorescence, infrared, Raman, EPR, NMR) imaging approaches (light and fluorescence microscopy, EM, super-resolution fluorescence microscopy, MRI), radioactive applications and calorimetric and fast kinetics techniques. The lectures discuss in details the physical basis and principles of each approach and the field of applications. The practicals are dedicated to extend the students’ knowledge and routine in the use of the different techniques. The practicals lay special emphasis on presenting not only the routine applications, but advanced uses of each technique. The limitations, as well as the artefacts that can be caused by improper experimental planning are highlighted.
Lectures
Practices
- 1. Introduction
- 2. Data bases, resources
- 3. Laser
- 4. Raman and infrared spectroscopy
- 5. UV-VIS spectroscopy
- 6. Fluorescence spectroscopy 1: steady state spectroscopy
- 7. Fluorescence spectroscopy 2: time dependent spectroscopy
- 8. Light microscopy
- 9. Image analysis
- 10. Rapid kinetic measurements: stopped-flow
- 11. Ultrasound
- 12. Revision
- 13. Preparation for the test/consultation
- 14. Test
Seminars
Reading material
Obligatory literature
Literature developed by the Department
http://www.biofizika.aok.pte.hu/tantargy/Molecular_Biophysics_Practicals_-_Biotechnology
Notes
http://www.biofizika.aok.pte.hu/tantargy/Molecular_Biophysics_Practicals_-_Biotechnology
Recommended literature
Conditions for acceptance of the semester
Maximum of 15 % absence allowed
Mid-term exams
A written test during the semester.
Making up for missed classes
Can be discussed with the course leader.
Exam topics/questions
http://www.biofizika.aok.pte.hu/tantargy/Molecular_Biophysics_Practicals_-_Biotechnology
Examiners
Instructor / tutor of practices and seminars
- Dr. Bugyi Beáta
- Dr. Bukovics Péter
- Dr. Huber Tamás
- Dr. Kengyel András Miklós
- Dr. Szabó-Meleg Edina
- Karádi Kristóf Kálmán
- Tempfliné Pirisi Katalin Erzsébet