Data
Official data in SubjectManager for the following academic year: 2021-2022
Course director
-
Dr. Zsuzsanna FEKETE
assistant professor,
Department of Biophysics -
Number of hours/semester
lectures: 0 hours
practices: 0 hours
seminars: 12 hours
total of: 12 hours
Subject data
- Code of subject: OSF-OPG-T
- 1 kredit
- Dentistry
- Optional modul
- spring
OSA-BZ1-T completed , OSA-MF1-T completed
Course headcount limitations
min. 5 – max. 24
Topic
Optogenetics refers to the intracellular use of photosensitive proteins that can be turned on or off using light to finely regulate specific biochemical processes.
This dynamically evolving revolutionary research method was first used by neuroscience in the early 2000s, but today, conquering several other fields of natural sciences, it can be found in basic biological, biophysical and biotechnological research as well as in many areas of medicine.
In order to demonstrate the extensive use of these proteins, we review the most well-known types of light-sensitive proteins, their mechanisms of action, and their biophysical characteristics. We then show, through a wealth of practical examples, how these proteins can be utilised. In addition to the methods used in basic research, we also focus on practical applications in medicine, primarily by presenting the latest advances in the treatment of certain diseases.
Lectures
Practices
Seminars
- 1. Introduction, general aspects of photo-responsive proteins
- 2. Opsins 1
- 3. Opsins 2
- 4. BLUF domains 1
- 5. BLUF domains 2
- 6. LOV domains 1
- 7. LOV domains 2
- 8. Fluorescent proteins
- 9. Cryptochromes 1
- 10. Cryptochromes 2
- 11. Biophysical aspects of the investigation of photo-responsive proteins
- 12. Test or presentation
Reading material
Obligatory literature
Literature developed by the Department
Will be published on Teams, Moodle or PotePedia.
Notes
Recommended literature
Conditions for acceptance of the semester
Maximum of 25 % absence allowed
Mid-term exams
Will be published on Teams, Moodle or PotePedia.
Making up for missed classes
None
Exam topics/questions
Will be published on Teams, Moodle or PotePedia.
Examiners
Instructor / tutor of practices and seminars
- Dr. Fekete Zsuzsanna
- Dr. Lukács András Szilárd
- Pécsi Ildikó