Biophysics - Lecture


Official data in SubjectManager for the following academic year: 2022-2023

Course director

Number of hours/semester

lectures: 28 hours

practices: 0 hours

seminars: 0 hours

total of: 28 hours

Subject data

  • Code of subject: OBA-106-E
  • 2 kredit
  • Biotechnology MSc
  • Basic modul
  • autumn


Exam course:


Course headcount limitations

min. 1


The Biophysics curse aims to introduce the students to the methods and applications, which are routinely used in both medical and pharmaceutical biotechnology. The principles of the state-of-the-art approaches and instrumentations are covered by the topics. The course presents diverse spectroscopic techniques (absorption, fluorescence, infrared, Raman, EPR, NMR) imaging approaches (light and fluorescence microscopy, EM, super-resolution fluorescence microscopy, MRI), radioactive applications and calorimetric and fast kinetics techniques. The lectures discuss in detail the physical basis and principles of each approach and the field of applications. The practicals are dedicated to extending the students' knowledge and routine in the use of the different techniques. The practicals lay special emphasis on presenting not only the routine applications but advanced uses of each technique. The limitations, as well as the artifacts that can be caused by improper experimental planning, are highlighted.


  • 1. Introduction: biophysics in biotechnology. - Dr. Bugyi Beáta
  • 2. Databases, resources. - Dr. Bugyi Beáta
  • 3. Electromagnetic waves, the electromagnetic spectrum. - Dr. Grama László
  • 4. The wave and particle nature of light. - Dr. Grama László
  • 5. Energy levels of atoms and molecules. - Dr. Lukács András Szilárd
  • 6. Laser. - Dr. Lukács András Szilárd
  • 7. UV-VIS spectroscopy. Circular dichroism (CD) spectroscopy. - Dr. Huber Tamás
  • 8. Infrared and Raman spectroscopy. - Dr. Bugyi Beáta
  • 9. Fluorescence spectroscopy, fluorescence parameters. Förster resonance energy transfer (FRET). - Dr. Huberné Dr. Barkó Szilvia
  • 10. Flow cytometry. Fluorescence activated cell sorting. - Dr. Huberné Dr. Barkó Szilvia
  • 11. The spin, nuclear magnetic resonance (NMR) spectroscopy. - Dr. Grama László
  • 12. Magnetic resonance imaging (MRI). - Dr. Grama László
  • 13. Basics of light microscopy and fluorescence microscopy. - Dr. Bugyi Beáta
  • 14. Advanced fluorescence microscopy 1. - Dr. Bugyi Beáta
  • 15. Advanced fluorescence microscopy 2. - Dr. Bugyi Beáta
  • 16. Image analysis. - Dr. Bugyi Beáta
  • 17. Rapid kinetic methods: stopped-flow, surface plasmon resonance (SPR). - Dr. Kengyel András Miklós
  • 18. Sound. Ultrasound. - Dr. Kengyel András Miklós
  • 19. Radioactivity, the interaction of radioactive radiations with the matter. - Dr. Szabó-Meleg Edina
  • 20. Dosimetry, detection of radioactive radiations. - Dr. Szabó-Meleg Edina
  • 21. Biological effects of radioactive radiations. - Dr. Kengyel András Miklós
  • 22. Gamma-camera, computed tomography (CT), single-photon emission computed tomography (SPECT), positron emission tomography (PET). - Dr. Kengyel András Miklós
  • 23. Thermodynamics: laws, thermodynamic potentials. - Dr. Lukács András Szilárd
  • 24. Calorimetric methods: differential scanning calorimetry (DSC), isothermal titration calorimetry (ITC). - Dr. Lukács András Szilárd
  • 25. Analytical separation techniques 1: sedimentation, electrophoresis. - Dr. Bukovics Péter
  • 26. Analytical separation techniques 2: chromatography. - Dr. Bukovics Péter
  • 27. Mass spectrometry. - Dr. Talián Csaba Gábor
  • 28. X-ray diffraction. - Dr. Talián Csaba Gábor



Reading material

Obligatory literature

Literature developed by the Department

Homepage of the Department of Biophysics:


Recommended literature

Conditions for acceptance of the semester

Maximum of 15 % absence allowed

Mid-term exams

Theory exams are scheduled in the exam period according to the rules of studies and examinations.

Making up for missed classes

The opportunity to make up for absence is not provided.

Exam topics/questions

Topics of the exam questions:
Electromagnetic waves
Quantum numbers, NMR, MRI
Energy levels of atoms and molecules
UV-VIS absorption spectroscopy
Fluorescence spectroscopy
Infrared and Raman spectroscopy
Rapid kinetic methods
Flow cytometry
Light and fluorescence microscopy
Advanced fluorescence microscopy
Image analysis
Sound, ultrasound
Gamma camera, computed tomography (CT), single-photon emission computed tomography (SPECT), positron emission tomography (PET)
Radioactivity, the interaction of radioactive radiations with matter
Biological effects of radioactive radiations, dosimetry
X-ray diffraction
Thermodynamics: laws and thermodynamic potentials
Calorimetry: differential scanning calorimetry (DSC), isothermal titration calorimetry (ITC)
Analytical separation techniques: sedimentation, electrophoresis
Analytical separation techniques: chromatographic techniques
Mass spectrometry


  • Dr. Bugyi Beáta
  • Dr. Bukovics Péter
  • Dr. Grama László
  • Dr. Kengyel András Miklós
  • Dr. Lukács András Szilárd
  • Dr. Szabó-Meleg Edina
  • Dr. Talián Csaba Gábor

Instructor / tutor of practices and seminars