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Education

Biotechnological Methods in Forensic Medicine

Data

Official data in SubjectManager for the following academic year: 2020-2021

Course director

Number of hours/semester

lectures: 12 hours

practices: 12 hours

seminars: 0 hours

total of: 24 hours

Subject data

  • Code of subject: OBF-BMF-T
  • 2 kredit
  • Biotechnology MSc
  • Optional modul
  • autumn
Prerequisites:

-

Course headcount limitations

min. 2 – max. 9

Topic

These days the importance of laboratory and molecular biology methods is obvious in the field of forensic medicine. These methods are used in the examination of different body fluids (blood, saliva, vaginal secretum). During the course we will discuss in details the methods used to analyze DNA (identifications, paternity testing): the significance of analyses of STRs, SNPs, and mitochodrial DNA. Besides the basic DNA methods we talk about new scientific approaches which are based on RNA and protein analyses (determination of course and time of death).

Lectures

  • 1. Sample collection, DNA extraction, specialities in Forensic Science - Dr. Poór Viktor Soma
  • 2. Forensic histology - Dr. Tóth Dénes
  • 3. Biology of short tandem repeats - Dr. Poór Viktor Soma
  • 4. The Chelex method - Fejes Vivien
  • 5. Next generation DNA sequencing - Dr. Poór Viktor Soma
  • 6. Identification of body fluids - Dr. Poór Viktor Soma
  • 7. Kinship analysis - Dr. Poór Viktor Soma
  • 8. Polymerase chain reaction - Dr. Poór Viktor Soma
  • 9. Forensic autopsy - Dr. Tóth Dénes
  • 10. Forensic applications of next generation sequencing - Dr. Poór Viktor Soma
  • 11. Diagnosis of drowning - Jánosa-Szűcs Dominika
  • 12. Examination - Dr. Poór Viktor Soma

Practices

  • 1. DNA extraction
  • 2. Forensic histology
  • 3. Polymerase chain reaction
  • 4. DNA extraction with Chelex
  • 5. Analysis of genetic profiles
  • 6. Analysis of genetic profiles
  • 7. Identification of biological stains
  • 8. Identification of biological stains
  • 9. Forensic autopsy
  • 10. Sequencing methods
  • 11. Diatom testing
  • 12. Consultation and exam

Seminars

Reading material

Obligatory literature

Literature developed by the Department

Notes

Recommended literature

Alan Gunn: Essential Forensic Biology,
Forensic DNA Typing, John M. Butler; Molecular Forensics, Ralph Rapley;
DNA: Forensic and Legal Applications Kobilinsky L.

Conditions for acceptance of the semester

Maximum of 25 % absence allowed

Mid-term exams

Written exam on the last practice

Making up for missed classes

Based on personal agreement

Exam topics/questions

Subjects of the exam are given out on the lectures

Examiners

Instructor / tutor of practices and seminars

  • Dr. Poór Viktor Soma
  • Dr. Tóth Dénes
  • Fejes Vivien
  • Jánosa-Szűcs Dominika
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