Molecular Targets – Signal Transduction - practice

Data

Official data in SubjectManager for the following academic year: 2020-2021

Topic

To demonstrate basic laboratory techniques in signal transduction.

Lectures

Practices

• 1. Measurement of intracellular Ca level using flow cytometry. T cell activation with anti-CD3.
• 2. Measurement of intracellular Ca level using flow cytometry. T cell activation with anti-CD3.
• 3. Measurement of intracellular Ca level using flow cytometry. T cell activation with anti-CD3.
• 4. Measurement of intracellular Ca level using flow cytometry. T cell activation with anti-CD3.
• 5. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
• 6. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
• 7. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
• 8. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
• 9. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
• 10. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
• 11. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
• 12. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
• 13. Signaling pathways of apoptosis (intrinsic and extrinsic). AnnexinV/propidium-iodode staining.
• 14. Signaling pathways of apoptosis (intrinsic and extrinsic). AnnexinV/propidium-iodode staining.
• 15. Signaling pathways of apoptosis (intrinsic and extrinsic). AnnexinV/propidium-iodode staining.
• 16. Signaling pathways of apoptosis (intrinsic and extrinsic). AnnexinV/propidium-iodode staining.
• 17. Signaling pathways of apoptosis (intrinsic and extrinsic). detection of activated Caspase-8, -9, -3.
• 18. Signaling pathways of apoptosis (intrinsic and extrinsic). detection of activated Caspase-8, -9, -3.
• 19. Signaling pathways of apoptosis (intrinsic and extrinsic). detection of activated Caspase-8, -9, -3.
• 20. Signaling pathways of apoptosis (intrinsic and extrinsic). detection of activated Caspase-8, -9, -3.
• 21. Signaling pathways of apoptosis (intrinsic and extrinsic). Analysis of the Bcl-2 protein family; changes of the mitochondrial membrane potential.
• 22. Signaling pathways of apoptosis (intrinsic and extrinsic). Analysis of the Bcl-2 protein family; changes of the mitochondrial membrane potential.
• 23. Signaling pathways of apoptosis (intrinsic and extrinsic). Analysis of the Bcl-2 protein family; changes of the mitochondrial membrane potential.
• 24. Signaling pathways of apoptosis (intrinsic and extrinsic). Analysis of the Bcl-2 protein family; changes of the mitochondrial membrane potential.
• 25. Analysis of the genomic and non-genomic glucocorticoid (GC) signaling pathways. Detection of the glucocorticoid-receptor using flow cytometry and microscopy.
• 26. Analysis of the genomic and non-genomic glucocorticoid (GC) signaling pathways. Detection of the glucocorticoid-receptor using flow cytometry and microscopy.
• 27. Analysis of the genomic and non-genomic glucocorticoid (GC) signaling pathways. Detection of the glucocorticoid-receptor using flow cytometry and microscopy.
• 28. Analysis of the genomic and non-genomic glucocorticoid (GC) signaling pathways. Detection of the glucocorticoid-receptor using flow cytometry and microscopy.

Seminars

Reading material

Obligatory literature

Gomperts: Signal Transduction

Literature developed by the Department

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Notes

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Recommended literature

Conditions for acceptance of the semester

Maximum of 15 % absence allowed

Mid-term exams

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Making up for missed classes

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Exam topics/questions

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Examiners

Instructor / tutor of practices and seminars

• Dr. Boldizsár Ferenc
• Dr. Prenek Lilla
• Kugyelka Réka