Data
Official data in SubjectManager for the following academic year: 2021-2022
Course director
-
Dr. Timea BERKI
professor,
Department of Immunology and Biotechnology -
Number of hours/semester
lectures: 14 hours
practices: 0 hours
seminars: 14 hours
total of: 28 hours
Subject data
- Code of subject: OBH-012-T
- 2 kredit
- Biotechnology MSc
- Specialty in Molecular Biotechnology modul
- autumn
Prerequisites:
OBA-106-E completed
Course headcount limitations
min. 5 – max. 50
Topic
The modern laboratory diagnostic methods, including molecular and immunological techniques will be discussed.
Lectures
- 1. Structure of a modern diagnostic laboratory, working areas, special devices and instrumentation. Sampling, biological information - Dr. Berki Timea
- 2. Basic terms of genetic diagnostics. Detection of mutations, polymorphisms. - Dr. Engelmann Péter András
- 3. Basic terms of immunologic detection methods. Antigen-antibody reactions - Dr. Berki Timea
- 4. Sensitivity, specificity of antigen-antibody reactions, detection methods - Dr. Berki Timea
- 5. Immonoassay types, laboratory automation - Dr. Berki Timea
- 6. Immunserological methods, autoantibody testing - Dr. Berki Timea
- 7. Immunserology, infection immunity - Dr. Berki Timea
- 8. Detection of allergen specific IgE, allergen purification methods, apitope testing - Dr. Berki Timea
- 9. HLA typing, molecular and serological methods - Dr. Berki Timea
- 10. HLA and disease assotiation, HLA-B27 typing - Dr. Berki Timea
- 11. Multiplex detection methods, biomarkers - Dr. Berki Timea
- 12. Cytokine, chemokine detection methods - Dr. Berki Timea
- 13. Detection of DNA modofications, methylation - Dr. Simon Diána
- 14. Molecular diagnostics of microbes, viruses - Dr. Engelmann Péter András
Practices
Seminars
- 1. Structure of a molecular genetic laboratory, working areas, special devices and instrumentation. Biological information.
- 2. Mutations, polymorphisms. The genetic code.
- 3. Mutations, polymorphisms. The genetic code.
- 4. Immunchemistry laboratory equipments, detection methods
- 5. Immunoassay detection methods.
- 6. Fluorescent detection methods, immunserology
- 7. autoimmunity detection methods
- 8. Diagnostics of systemic autoimmune diseases
- 9. HLA and diseases
- 10. Phage dysplay library in molecular diagnostics
- 11. Protein chemistry
- 12. Molecular testing in oncology.
- 13. Laboratory diagnostics of allergy
- 14. Methodology of the molecular diagnostic procedures.
Reading material
Obligatory literature
Literature developed by the Department
www.medbiotech.com
Notes
Recommended literature
Conditions for acceptance of the semester
Maximum of 25 % absence allowed
Mid-term exams
We will use the medtraining.eu website to take test questions for the students from the materilas of the lectures and seminars.
Making up for missed classes
None
Exam topics/questions
www.medtraining.eu
Examiners
- Dr. Berki Timea
- Dr. Simon Diána
Instructor / tutor of practices and seminars
- Bodó Kornélia
- Böröcz Katalin
- Dr. Engelmann Péter András
- Dr. Simon Diána