Data
Official data in SubjectManager for the following academic year: 2021-2022
Course director
-
Dr. Péter BALOGH
professor,
Department of Immunology and Biotechnology -
Number of hours/semester
lectures: 0 hours
practices: 28 hours
seminars: 0 hours
total of: 28 hours
Subject data
- Code of subject: OBH-007-G
- 2 kredit
- Biotechnology MSc
- Specialty in Medical Biotechnology modul
- autumn
Prerequisites:
OBH-007-E parallel
Course headcount limitations
min. 1 – max. 25
Topic
Thje purpose of the subject is to provide laboratory training in basic embryological and stem-cell related analytical methods.
Lectures
Practices
- 1. Alkaline phosphatase assay of JAR cells I.
- 2. Alkaline phosphatase assay of JAR cells II.
- 3. Invertebrate regeneration I
- 4. Invertebrate regeneration II
- 5. Histological analysis of invertebrate regeneration I
- 6. Histological analysis of invertebrate regeneration II
- 7. Isolation of eGFP bone marrow cells and hematopoietic reconstitution I
- 8. Isolation of eGFP bone marrow cells and hematopoietic reconstitution II
- 9. Visit to the IVF laboratory I
- 10. Visit to the IVF laboratory II.
- 11. Isolation of organs from mid-term mouse embryos I.
- 12. Isolation of organs from mid-term mouse embryos II.
- 13. In situ and ex vivo photoconversion of Kik photoprotein I.
- 14. Isolation of organs from mid-term mouse embryos II.
- 15. Phenotypic analysis of fetal organ-derived cultures: neural, liver, cardiac I.
- 16. Phenotypic analysis of fetal organ-derived cultures: neural, liver, cardiac II.
- 17. Analysis of hematopoietic stem cell engraftment in eGFP-transplanted mice I.
- 18. Analysis of hematopoietic stem cell engraftment in eGFP-transplanted mice II.
- 19. Isolation pf preimplantation mouse embryos and initiation of embryonic stem cell cultures I.
- 20. Isolation pf preimplantation mouse embryos and initiation of embryonic stem cell cultures II.
- 21. Passage of hatching embryos and expansion of eSC culture I.
- 22. Passage of hatching embryos and expansion of eSC culture II.
- 23. AP analysis of eSC cultures I.
- 24. AP analysis of eSC cultures II.
- 25. FACS sorting of tumor-inducing cells from Bc-DLFL.1 lymphoma cells I.
- 26. FACS sorting of tumor-inducing cells from Bc-DLFL.1 lymphoma cells II.
- 27. Consultation
- 28. Consultation
Seminars
Reading material
Obligatory literature
n/a
Literature developed by the Department
handouts and protocols provided at each practice
Notes
n/a
Recommended literature
n/a
Conditions for acceptance of the semester
Maximum of 15 % absence allowed
Mid-term exams
n/a
Making up for missed classes
none
Exam topics/questions
n/a
Examiners
Instructor / tutor of practices and seminars
- Dr. Balogh Péter
- Dr. Engelmann Péter András