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Supervisor: KÁTAI, Emese
Co-supervisor: Dr. PÁL, József
The protein post-translational modification called O-Glycosylation (or O-GlcNAc) is a special process; in contrast to N-glycosylation, O-GlcNAc is reversible, occurs in the cytoplasm and in the nucleus, and similarly to phosphorylation, targets Ser/Thr side-chains of the proteins. More than 500 proteins has been shown so far to carry an O-GlcNAc moiety. It is involved in the regulation of several intracellular mechanism; carbohydrate metabolism, cell cycle, malignant transformation, etc.
It has been shown that O-GlcNAc is increased upon stress which seems to be a natural adaptation mechanism helping the cells’ survival. Blocking O-GlcNAc by inhibitors caused lower viability and increased apoptosis, following stress such as hypoxia or heat shock. The aim of this study is to characterize the dynamic change of O-GlcNAc level and its impact on cell survival in neuronal cells and in the brain tissue of experimental animals. Learning more about O-GlcNAc’s role in the adaptation of neuronal cells could help to improve therapeutic strategies in brain injuries including stroke.