Official data in SubjectManager for the following academic year: 2020-2021
Dr. Attila János MISETA (email@example.com), professor
Institute of Laboratory Medicine
Code of subject: OAK-KBK-T | 2 credit | General Medicine | Clinical module | autumn
Prerequisites: OAA-OBA-T completed , OAP-PA2-T completed , OAP-KO2-T completedExam course: no
14 lectures + 14 practices + 0 seminars = total of 28 hours
min. 5 – max. 100 person
The aim of clinical biochemistry is to improve the ability of medical students to integrate information provided by the clinical laboratory with other clinical diagnostic disciplines and thus, to form an accurate diagnosis. We will give you a guideline how to request and interpret laboratory tests to diagnose, evaluate the prognosis and monitor the therapy of the patients. The subject "Clinical Biochemistry" requires your existing knowledge in biochemistry and pathology. We will teach you how to select from a variety of laboratory parameters, provide you with a strategy on how and when to order these tests, and help you to develop a molecular approach to medicine. Clinical biochemistry includes classic subfields such as clinical chemistry, hematology, hemostaseology, immunology, however the lectures and seminars will emphasize that instead of sub-categories it is more important to treat patients and their diseases with the educated use of the complete arsenal of the clinical laboratory.
W.J. Marshall, S.K. Bangert: Clinical Chemistry, 7th ed., Mosby Co, 2012, ISBN 9780723437048
G.J. Beckett, S.W. Walker, P. Rae, P. Ashby: Lecture Notes on Clinical Biochemistry, 8th ed., Wiley-Blackwell Co., 2013. ISBN 978-1-4443-9475-7
A. Gaw, M.J. Murphy, R.A. Cowan, D.St.J. O'Reilly, M.J. Stewart, J. Shepherd: Clinical Biochemistry, An Illustrated Colour Text, 4th ed., Churchill Livingstone Elsevier 2008
Attending the lectures and practices is obligatory. Absences up to 25% are accepted.
Attending the practices is obligatory. If someone misses a practice for any reason, he/she is asked to attend the practice in question given to another group.
Questions for the oral examination
1. Purpose of laboratory test requests (screening, diagnosis, differential-diagnosis, validation, monitoring).
2. Patient preparation before sampling. Proper method to carry out blood (venous, capillary) and urine collection. Type of tubes.
3. Reference values and ranges, specificity, sensitivity and predictive value of laboratory tests.
4. Interpretation of laboratory results. Biological and analytical factors influencing the results.
5. Bedside/point of care tests (POCT) and their informational value.
6. Disorders of water and sodium homeostasis.
7. Disorders of potassium balance.
8. Laboratory findings in metabolic type changes of the acid/base balance.
9. Laboratory findings in respiratory type changes of the acid/base balance.
10. Laboratory approaches for the detection of disorders in calcium, magnesium and phosphate homeostasis.
11. Clinical biochemistry of osteoporosis. Laboratory tests to assess joint and bone disorders.
12. Laboratory analysis of plasma proteins.
13. Laboratory analysis of plasma enzymes.
14. Disturbances of non-immunoglobulin plasma proteins.
15. Disorders of immunoglobulins and paraproteins.
16. Assessment of acute phase proteins, diagnosis and monitoring of sepsis.
17. Laboratory findings in inflammatory disorders.
18. Laboratory diagnosis of malignant hematologic disorders; complete blood count and flow cytometry
19. Laboratory diagnostic approaches in anemias.
20. Hemoglobinopathies: disorders of the porphyrin metabolism.
21. Laboratory findings in the disorders of iron metabolism.
22. Blood coagulation: cellular components (platelets, endothelial cells) and their laboratory analysis.
23. Blood coagulation: functional tests (PT, APTT, TT, fibrinogen, D-dimer).
24. Laboratory monitoring of anti-coagulant therapies.
25. Current analytical protocol to detect acute myocardial infarction; international recommendations.
26. Laboratory monitoring the therapy of myocardial infarction. Laboratory approaches in chronic heart failure (BNP, proBNP, electrolytes).
27. Laboratory diagnostics of the striated muscle diseases.
28. Diagnostic criteria of diabetes mellitus (WHO criteria). Laboratory monitoring of patients with diabetic history.
29. Clinical biochemistry of hypoglycemia and hyperglycemia.
30. Lipids and lipoproteins in the blood plasma.
31. Laboratory tests that predict hepatic disorders.
32. Laboratory diagnostics of alcoholic liver damage. Laboratory tests to assess liver fibrosis.
33. Disorders of bilirubin metabolism.
34. Laboratory diagnosis of acute pancreatitis.
35. Laboratory diagnosis of chronic pancreatitis.
36. laboratory diagnosis and monitoring of malignant and inflammatory bowel diseases.
37. Laboratory diagnosis of acute renal diseases.
38. Laboratory diagnosis and monitoring of chronic renal diseases.
39. Laboratory findings in proteinuria and hematuria.
40. Laboratory assessment of increased serum uric acid levels.
41. Laboratory tests of the cerebrospinal fluid and other body fluids.
42. Pre-analytical considerations of the hormone tests.
43. Clinical biochemistry of the hypothalamus, hypophysis.
44. Laboratory assessment of the thyroid function.
45. Clinical biochemistry of the disorders of adrenal medulla/cortex.
46. Clinical biochemistry of the disorders of the human reproductive system.
47. The most important non-specific laboratory tests that suggest the presence of malignant diseases.
48. Tumor markers and their informational value in the clinical laboratory practice.
49. Molecular biology applications in the practice of clinical laboratories.
50. Therapeutic drug monitoring (TDM).
51. Toxicology tests in the clinical laboratory.
Questions for evaluating laboratory results
1. Normal results
2. Oral or s.c. anti-coagulated patient
3. Respiratory acidosis
4. Metabolic acidosis
5. Acute myocardial infarct
7. Acute renal failure
8. Chronic renal disease (dialyzed patient)
9. Acute liver disease
10. Liver failure
11. Bile obstruction
12. Acute pancreatitis
13. Myeloma multiplex
14. Leukemia (blood cell count)
15. Leukemia (blood smear)
17. Iron deficiency anemia
19. Adrenal crisis
20. Acute inflammation
23. Malignancy (tumormarkers)