Data
Official data in SubjectManager for the following academic year: 2020-2021
Course director
-
dr. Kornélia FARKAS-BORBÁS
assistant professor,
Institute of Bioanalysis -
Number of hours/semester
lectures: 6 hours
practices: 6 hours
seminars: 0 hours
total of: 12 hours
Subject data
- Code of subject: OSF-EPR-T
- 1 kredit
- Dentistry
- Optional modul
- both semesters
OSA-BZ1-T completed
Course headcount limitations
min. 1 – max. 6
Topic
The aim of the course is to learn the usual techniques in EPR spectroscopy on different samples, and evaluation of the results obtained.
The electron paramagnetic resonance spectroscopy is a method similar to NMR, but deals with the properties of electron spin resonance. The technique provides data on the molecular structure and dynamics of various, e.g. biological systems.
The investigation of paramagnetic materials, like free radicals, metals, and special spin-labels help to describe biological systems, e.g. membranes, proteins.
Lectures
- 1. The basics of EPR Spectroscopy - Dr. Dergez Tímea
- 2. The basic features of EPR Spectrometer - Dr. Dergez Tímea
- 3. Spin labels and labeling methods - Dr. Dergez Tímea
- 4. Free radicals - spin trapping - Dr. Dergez Tímea
- 5. Membrane structures and their labeling - Borbásné Dr. Farkas Kornélia
- 6. Other biological structures and their labeling - Borbásné Dr. Farkas Kornélia
Practices
- 1. Way of registration, registration and analyze of an EPR spectra
- 2. Measurements of membrane dynamics 1.
- 3. Measurements of membrane dynamics 2.
- 4. Labeling and measuring of other biological structures 1.
- 5. Labeling and measuring of other biological structures 2.
- 6. Spin trapping
Seminars
Reading material
Obligatory literature
Literature developed by the Department
Notes
Recommended literature
1. C. P. Poole: Electron Spin Resonance, John Wiley & Sons, New York, 1983
2. Hoppe, N., Lohmann, W., Marke, H., Ziegler, H.: Biophysics, Springer-Verlag, Berlin, 1987
Conditions for acceptance of the semester
Oral exam
Mid-term exams
Making up for missed classes
Consultation
Exam topics/questions
Examiners
Instructor / tutor of practices and seminars
- Borbásné Dr. Farkas Kornélia
- Dr. Dergez Tímea