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Education

Use of the EPR Spectroscopy on Biological Structures

Data

Official data in SubjectManager for the following academic year: 2020-2021

Course director

Number of hours/semester

lectures: 6 hours

practices: 6 hours

seminars: 0 hours

total of: 12 hours

Subject data

  • Code of subject: OXF-EPR-h-T
  • 1 kredit
  • General Medicine
  • Optional modul
  • both semesters
Prerequisites:

ODA-BI1-T completed

Course headcount limitations

min. 1 – max. 6

Topic

The aim of the course is to learn the usual techniques in EPR spectroscopy on different samples, and evaluation of the results obtained.
The electron paramagnetic resonance spectroscopy is a method similar to NMR, but deals with the properties of electron spin resonance. The technique provides data on the molecular structure and dynamics of various, e.g. biological systems.
The investigation of paramagnetic materials, like free radicals, metals, and special spin-labels help to describe biological systems, e.g. membranes, proteins.

Lectures

  • 1. The basics of EPR Spectroscopy - Dr. Dergez Tímea
  • 2. The basic features of EPR Spectrometer - Dr. Dergez Tímea
  • 3. Spin labels and labeling methods - Dr. Dergez Tímea
  • 4. Free radicals - spin trapping - Dr. Dergez Tímea
  • 5. Membrane structures and their labeling - Borbásné Dr. Farkas Kornélia
  • 6. Other biological structures and their labeling - Borbásné Dr. Farkas Kornélia

Practices

  • 1. Way of registration, registration and analyze of an EPR spectra
  • 2. Measurements of membrane dynamics 1.
  • 3. Measurements of membrane dynamics 2.
  • 4. Labeling and measuring of other biological structures 1.
  • 5. Labeling and measuring of other biological structures 2.
  • 6. Spin trapping

Seminars

Reading material

Obligatory literature

Literature developed by the Department

Notes

Recommended literature

1. C. P. Poole: Electron Spin Resonance, John Wiley & Sons, New York, 1983
2. Hoppe, N., Lohmann, W., Marke, H., Ziegler, H.: Biophysics, Springer-Verlag, Berlin, 1987

Conditions for acceptance of the semester

Oral exam

Mid-term exams

Making up for missed classes

Consultation

Exam topics/questions

Examiners

Instructor / tutor of practices and seminars

  • Borbásné Dr. Farkas Kornélia
  • Dr. Dergez Tímea
Rólunk