Urinary Steroid Profiles

Data

Official data in SubjectManager for the following academic year: 2020-2021

Course director

Number of hours/semester

lectures: 6 hours

practices: 6 hours

seminars: 0 hours

total of: 12 hours

Subject data

  • Code of subject: OXF-VSP-h-T
  • 1 kredit
  • General Medicine
  • Optional modul
  • both semesters
Prerequisites:

-

Course headcount limitations

min. 1 – max. 15

Available as Campus course for 1 fő számára. Campus-karok: TTK

Topic

The participants are going to be familiarised theoretically and practically with:
- pre-treatment of urine sample
- qualitative and quantitative analysis of 24 androgen, progesterone and corticoid metabolites by gas chromatography using mass selective detector
- evaluation of urinary steroid profiles

Lectures

  • 1. Urinary steroid metabolites - Dr. Bufa Anita
  • 2. Sample pre-treatment of urine - Dr. Bufa Anita
  • 3. Gas chromatography-Mass spectrometry - Dr. Bufa Anita
  • 4. Qualitative analysis of urinary steroid metabolites - Dr. Bufa Anita
  • 5. Quantitative analysis of urinary steroid metabolites - Dr. Bufa Anita
  • 6. Evaluation of urinary steroid profile - Dr. Bufa Anita

Practices

  • 1. Solid-phase extraction of urinary steroid metabolites
  • 2. Enzymatic hydrolysis of urinary steroid metabolites
  • 3. Derivatization of urinary steroid metabolites
  • 4. Chromatographic cleaning
  • 5. Determination of urinary steroid profiles by GC-MS technic
  • 6. Qualitative and quantitative analysis of urinary steroid metabolites

Seminars

Reading material

Obligatory literature

Literature developed by the Department

It is going to be available on the website.

Notes

Recommended literature

Conditions for acceptance of the semester

Maximum of 15 % absence allowed

Mid-term exams

After the course a written summary.

Making up for missed classes

Consultation with course director is required. The necessary information is going to be presented on the course.

Exam topics/questions

The necessary information is going to be presented on the course.

Examiners

Instructor / tutor of practices and seminars

  • Dr. Bufa Anita