Data
Official data in SubjectManager for the following academic year: 2020-2021
Course director
-
Dr. Ferenc KILÁR
professor,
Institute of Bioanalysis -
Number of hours/semester
lectures: 28 hours
practices: 0 hours
seminars: 0 hours
total of: 28 hours
Subject data
- Code of subject: OBA-109-E
- 2 kredit
- Biotechnology MSc
- Basic modul
- spring
OBA-108-E completed
Exam course:no
Course headcount limitations
min. 1
Topic
The purpose of this subject is to describe and characterize proteins, which are the most important components in the cell-function. Analysis of the proteome with molecular biology, bioinformatics and biostatistical methods. Structure-function relationships in proteins. Protein associates. Methodology to study the structure of proteins (NMR, mass spectrometry). Purification of proteins. Bioanalysis of proteins.
Lectures
- 1. Basic description of protein structure - Dr. Kilár Ferenc
- 2. Basic description of protein structure - Dr. Kilár Ferenc
- 3. Structure-function relationships in proteins - Dr. Kilár Ferenc
- 4. Structure-function relationships in proteins - Dr. Kilár Ferenc
- 5. Domain-multidomain setups and functions - Dr. Kilár Ferenc
- 6. Domain-multidomain setups and functions - Dr. Kilár Ferenc
- 7. Structure investigation with advanced methods. NMR of proteins - Dr. Kilár Ferenc
- 8. Structure investigation with advanced methods. NMR of proteins - Dr. Kilár Ferenc
- 9. Crystallization of proteins. - Dr. Kilár Ferenc
- 10. Crystallization of proteins. - Dr. Kilár Ferenc
- 11. X-ray crystallography - Dr. Kilár Ferenc
- 12. X-ray crystallography - Dr. Kilár Ferenc
- 13. Dynamics of amino acid networks - Dr. Kilár Ferenc
- 14. Dynamics of amino acid networks - Dr. Kilár Ferenc
- 15. Serum proteins - Dr. Kilár Ferenc
- 16. Serum proteins - Dr. Kilár Ferenc
- 17. Protein associates - Dr. Kilár Ferenc
- 18. Protein associates - Dr. Kilár Ferenc
- 19. Mass spectrometry in proteomics - Dr. Kilár Ferenc
- 20. Mass spectrometry in proteomics - Dr. Kilár Ferenc
- 21. Protein-protein interactions - Dr. Kilár Ferenc
- 22. Protein-protein interactions - Dr. Kilár Ferenc
- 23. Bioanalytical methods to examine protein associates - Dr. Kilár Ferenc
- 24. Bioanalytical methods to examine protein associates - Dr. Kilár Ferenc
- 25. Surface interaction methods - Dr. Kilár Ferenc
- 26. Surface interaction methods - Dr. Kilár Ferenc
- 27. Signal transduction of proteins - Dr. Kilár Ferenc
- 28. Signal transduction of proteins - Dr. Kilár Ferenc
Practices
Seminars
Reading material
Obligatory literature
Literature developed by the Department
Notes
Recommended literature
F. Lottspeich and J. W. Engels (eds.): Bioanalytics. Bioanalytical Methods and Concepts in Biochemistry and Molecular Biology, Wiley-VCH, Weinheim, Germany, 2018
D. Liu and F-Y. Wan (eds.): Advances in Computational Intelligence: Theory & Applications,
World Scientific, Hackensack, 2006
Petsko, Gregory A./ Ringe, Dagmar: Protein Structure and Function, Sinauer Associates Inc., 2004
Buxbaum, Engelbert: Fundamentals of Protein Structure and Function, Springer, 2007
Conditions for acceptance of the semester
Maximum of 25 % absence allowed
Mid-term exams
Retake is not possible.
Making up for missed classes
According to the Faculty rules
Exam topics/questions
Website of the Institute of Bioanalysis
http://aok.pte.hu/en/egyseg/oktatasianyagok/160
Examiners
- Dr. Kilár Ferenc