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Capillary Electrophoresis in Laboratory Diagnostics

Data

Official data in SubjectManager for the following academic year: 2020-2021

Course director

Number of hours/semester

lectures: 12 hours

practices: 0 hours

seminars: 0 hours

total of: 12 hours

Subject data

  • Code of subject: OSF-KFO-T
  • 1 kredit
  • Dentistry
  • Optional modul
  • autumn
Prerequisites:

-

Course headcount limitations

min. 5 – max. 50

Available as Campus course for 50 fő számára. Campus-karok: ETK TTK

Topic

Capillary electrophoresis in laboratory diagnostic processes are considered to be the most modern separation techniques. Its application in health sciences is obvious. The course deals with the theoretical background and application of the microfluidic methods. Design of capillary electrophoretic experiments. Proteins and small molecules. Electrophoresis of cells. Special applications, chiral separations, microbiological applications.

Lectures

  • 1. Evolution of separation methods - Fenyvesiné Dr. Páger Csilla
  • 2. Development of new electrophoretic methods - Fenyvesiné Dr. Páger Csilla
  • 3. Theory of electrophoresis - Fenyvesiné Dr. Páger Csilla
  • 4. Separation modes in electrophoresis, Zone electrophoresis - Fenyvesiné Dr. Páger Csilla
  • 5. Isoelectric focusing theory, IEF methodology, sandwich injection - Fenyvesiné Dr. Páger Csilla
  • 6. Isotachophoresis - Fenyvesiné Dr. Páger Csilla
  • 7. Gel electrophoresis - Fenyvesiné Dr. Páger Csilla
  • 8. High resolution analysis of macromolecules - Fenyvesiné Dr. Páger Csilla
  • 9. Chiral analysis of drugs - Fenyvesiné Dr. Páger Csilla
  • 10. Lab-on-a-chip methodology - Fenyvesiné Dr. Páger Csilla
  • 11. Application in laboratory diagnostics - Fenyvesiné Dr. Páger Csilla
  • 12. Coupling with mass spectrometry - Fenyvesiné Dr. Páger Csilla

Practices

Seminars

Reading material

Obligatory literature

Literature developed by the Department

http://aok.pte.hu/bioanalitika/Oktatas/Oktatas.html

Notes

Recommended literature

James Landers (ed.): Handbook of Capillary Electrophoresis, CRC Press, 1994
Ann Van Schepdael: Microchip Capillary Electrophoresis Protocols, Springer, 2015

Conditions for acceptance of the semester

Maximum of 25 % absence allowed

Mid-term exams

Written examination within the last lecture.

Making up for missed classes

According to the faculty rules.

Exam topics/questions

Discussion of practical questions after home work.

Examiners

Instructor / tutor of practices and seminars

Rólunk