Modern Analitical Devices

Data

Official data in SubjectManager for the following academic year: 2022-2023

Topic

Course lectures will present current analytical procedures relevant to biotechnology, including analytical procedures, their basic theories, molecular interactions, data collection methods. During course practices, the students will familiarize themselves with the molecular bases of modern analytical procedures, will experience their advantages, disadvantages, and limitations during actual work with these devices. In addition, practices give the opportunity for students to perform molecular coupling techniques, gold nanoparticles, surface utilization, become familiar with devices, and test their basic characteristics. During this course, students gain important interdisciplinary knowledge on the verge of biotechnology/nanotechnology/pharmaceutical technology and also get insight into the analytics of natural and artificial vesicles. Such knowledge will prove to be helpful once searching for job opportunities in the labor market, in either basic or applied research.

Lectures

• 1. Immobilized biomolecules in bioassay - Dr. Secenji Aleksandar
• 2. Fluorescence techniques - Dr. Secenji Aleksandar
• 3. Immunoassay methods - Dr. Secenji Aleksandar
• 4. Immunological stripe tests - Dr. Secenji Aleksandar
• 5. Biosensors - Dr. Secenji Aleksandar
• 6. Biochips - Dr. Secenji Aleksandar
• 7. Nanoclusters and colloids in bioanalysis - Dr. Secenji Aleksandar
• 8. AFM - Dr. Secenji Aleksandar
• 9. Complex biological analysis - Dr. Secenji Aleksandar
• 10. Automated analytical systems: FIA - Dr. Secenji Aleksandar
• 11. Automated analytical systems: SIA - Dr. Secenji Aleksandar
• 12. Automated analytical systems: TAS - Dr. Secenji Aleksandar
• 13. Automated analytical systems: µTAS - Dr. Secenji Aleksandar
• 14. Automated analytical systems: ELISA - Dr. Secenji Aleksandar

Practices

• 1. 1.gyakorlat
• 2. 1.gyakorlat
• 3. 1.gyakorlat
• 4. 1.gyakorlat
• 5. 2.gyakorlat
• 6. 2.gyakorlat
• 7. 2.gyakorlat
• 8. 2.gyakorlat
• 9. 3.gyakorlat
• 10. 3.gyakorlat
• 11. 3.gyakorlat
• 12. 3.gyakorlat
• 13. 4.gyakorlat
• 14. 4.gyakorlat
• 15. 4.gyakorlat
• 16. 4.gyakorlat
• 17. 5.gyakorlat
• 18. 5.gyakorlat
• 19. 5.gyakorlat
• 20. 5.gyakorlat
• 21. 6.gyakorlat
• 22. 6.gyakorlat
• 23. 6.gyakorlat
• 24. 6.gyakorlat
• 25. 7.gyakorlat
• 26. 7.gyakorlat
• 27. 7.gyakorlat
• 28. 7.gyakorlat

Seminars

Reading material

Obligatory literature

1. Thomas G .M. Schalkhammer (ed): Analytical Biotechnology, 2002 Springer Basel AG, ISBN: 978-3-7643-6589-9
2. Friedrich Lottspeich and Joachim Engels (eds): Bioanalytics Analytical Methods and Concepts in Biochemistry and Molecular Biology, 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Boschstr. 12, 69469 Weinheim, Germany, ISBN: 978-3-527-69444-0

Literature developed by the Department

Notes

Recommended literature

3. Wolfgang Fritzsche and Jürgen Popp (vol. eds): Optical Nano- and Microsystems for Bioanalytics, Springer-Verlag Berlin Heidelberg 2012, ISBN: 978-3-642-25497-0

Conditions for acceptance of the semester

Maximum of 15 % absence allowed

Mid-term exams

one written, and one oral exam in announced time

Making up for missed classes

one absence, one practice on last occasion

Exam topics/questions

1. Immobilized biomolecules in bioassay
2. Fluorescence techniques
3. Immunoassay methods
4. immunological strip tests
5. Biosensors
6. Biochips
7. Nanoclusters and colloids in bioanalysis
8. Atomic force microscopy
9. Complex biological analysis
10. Automated analytical systems: FIA
11. Automated analytical systems: SIA
12. Automated analytical systems: TAS
13. Automated analytical systems: µTAS
14. Automated analytical systems: ELISA

Examiners

• Dr. Secenji Aleksandar
• Dr. Vörös-Horváth Barbara

Instructor / tutor of practices and seminars

• Dr. Vörös-Horváth Barbara