Medicinal Plant Biotechnology - practice

Data

Official data in SubjectManager for the following academic year: 2021-2022

Topic

Medicinal plant biotechnology is one of the most interesing field of pharmeceutical sciences. The plant secondary metabolites possess very interesting and important biological and pharmacological effects. This course gives an overview of medicinal plant biotechnology describing the whole spectrum of biotecnological tools from cell culture techniques to the biosynthesis and accumulation of pharmaceutical compounds in the plants, genetic transformation, metabolic engineering, metabolomics, processes for the production of authenticated raw materials and quality control of materials for their safe and efficacious use. During practices, students make molecular biological and cell culture investigations.

Lectures

Practices

• 1. Fire and accident safety education. Introduction of general methods of analysis for drugs of plant origin I.
• 2. Introduction of general methods of analysis for drugs of plant origin II.
• 3. The in vitro production of pharmaceutically effective metabolites I.: Carbohydrates
• 4. The in vitro production of pharmaceutically effective metabolites I.: Carbohydrates
• 5. The in vitro production of pharmaceutically effective metabolites II.: phenoloids (anthraglycosids, anthranoids)
• 6. The in vitro production of pharmaceutically effective metabolites II.: phenoloids (anthraglycosids, anthranoids)
• 7. The in vitro production of pharmaceutically effective metabolites III.: phenoloids (anthocyanins, proanthocyanidin, tannins) Short test 1.
• 8. The in vitro production of pharmaceutically effective metabolites III.: phenoloids (anthocyanins, proanthocyanidins, tannins)
• 9. The in vitro production of pharmaceutically effective metabolites IV.: phenoloids (flavonoids) Short test 2.
• 10. The in vitro production of pharmaceutically effective metabolites IV.: phenoloids (flavonoids)
• 11. The in vitro production of pharmaceutically effective metabolites V.: terpenoids (triterpenes)
• 12. The in vitro production of pharmaceutically effective metabolites V.: terpenoids (triterpenes)
• 13. The in vitro production of pharmaceutically effective metabolites VI.: terpenoids (essential oils) Short test 3.
• 14. The in vitro production of pharmaceutically effective metabolites VI.: terpenoids (essential oils)
• 15. The in vitro production of pharmaceutically effective metabolites VII.: Fixed oils and fats
• 16. The in vitro production of pharmaceutically effective metabolites VII.: Fixed oils and fats
• 17. The in vitro production of pharmaceutically effective metabolites VIII.: Azotoids. (e.g. alkaloids) Short test 4.
• 18. The in vitro production of pharmaceutically effective metabolites VIII.: Azotoids. (e.g. alkaloids).
• 19. Introduction to plant-microtechnical methods. Cell, tissue and organ cultures: the propagation and the metabolism of isolated plant cells and tissues.
• 20. Conditions to culturing plant cells: the different types of plant cell, tissue and organ cultures. Maintaining the cultures.
• 21. Gene transfer techniques. The genetic code, gene transfer (indirect gene transfers; Agrobacterium mediated gene transfer; virus vectors, and direct gene transfer methods).
• 22. Enhancing agent production in tissue cultures: with hormonal regulation, elicitation, biotransformation and with microbiological gene transformation
• 23. Final Test
• 24. Final Test
• 25. Students' presentations.
• 26. Students' presentations.
• 27. Consultation.
• 28. Consultation.

Seminars

Reading material

Obligatory literature

Literature developed by the Department

Notes

Recommended literature

Chandra S, Lata H, Varma A (eds.): Biotechnology for medicinal plants. Springer, New York, 2013.
Kayser O, Quax W (eds.): Medicinal plant biotechnology. Vol. 1 and 2., Wiley, Weinheim, 2009.

Conditions for acceptance of the semester

There is no possibility to miss the practices.

Mid-term exams

Students need to write one written exams during semester. Furthermore, students have to take a presentation based on an english article related to medicinal plant biotechnology.

Making up for missed classes

There is no possibility to make up for the missed practices.

Exam topics/questions

Not applicable.

Examiners

Instructor / tutor of practices and seminars

• Dr. Ács Kamilla