Supervisor: NAGYNÉ, Kiss Gyöngyi
White adipose tissue is an endocrine organ producing several hormones (e.g leptin, adiponectin, resistin etc.) and cytokines (e.g TNF-α, MCP-1, IL-6 etc.) involved in the regulation of tissue insulin sensitivity. Obesity-associated decrease in tissue insulin sensitivity, also called insulin resistance, is a major risk factor for type 2 diabetes and cardiovascular diseases.
Lysophosphatidic acid (LPA) is a phospholipid mediator that regulates several physiological responses ranging from cell proliferation and differentiation to cell migration and survival, via specific cell membrane and nuclear receptors. Recent studies showed that white adipose tissue secretes a significant amount of LPA and also the key enzyme of LPA production, autotaxin. The expression of ATX is increased in the adipose tissue of obese insulin-resistant individuals and mice. Furthermore, it has been shown that LPA decreased pancreatic insulin secretion and glucose tolerance. By using isolated mouse adipocytes and LPA receptor knockout mice models we aim to investigate the effect of LPA on the production of certain hormones (leptin, adiponectin, resistin) and cytokines (TNF-α, MCP-1, IL-6) involved in the regulation of tissue insulin sensitivity, and also the LPA receptors and intracellular signaling pathways involved in this process.