Biochemistry

Data

Official data in SubjectManager for the following academic year: 2020-2021

Topic

The subject gives an overview of the molecular processes occurring in living organisms from two major aspects.
On the one hand, following the subject "Introduction to Biochemistry", it describes the metabolism of the four major types of biomolecules (carbohydrates, lipids, amino acids/proteins and nucleotides/nucleic acids) along with the indirect enzymatic regulation of these processes.
On the other hand, built on the subject "Molecular Cell Biology", accounts for the molecular processes responsible for the transmission of information within the living organisms from molecular biological (DNA-RNA-protein-metabolite) as well as hormonal point of view.
The subject lies down the foundations of the subjects Medical Biochemistry, Pharmacology and Clinical Chemistry.

Lectures

• 1. Introduction - Dr. Veres Balázs
• 2. The pentose phosphate pathway - Dr. Veres Balázs
• 3. Gluconeogenesis in animal cells - Dr. Kovács Krisztina
• 4. Glycogen synthesis and degradation - Dr. Veres Balázs
• 5. Regulation of glycogen synthesis and degradation - Dr. Veres Balázs
• 6. Regulation and disorders of carbohydrate metabolism - Dr. Kovács Krisztina
• 7. Fatty acid synthesis (with elongation and desaturation) - Dr. Gallyas Ferenc
• 8. Synthesis of complex lipids - Dr. Gallyas Ferenc
• 9. Structure and biological activities of steroids - Dr. Gallyas Ferenc
• 10. Synthesis of cholesterol and ketone bodies - Dr. Gallyas Ferenc
• 11. Regulation and disorders of lipid metabolism - Dr. Gallyas Ferenc
• 12. Amino acid metabolism; the fate of the amino group - Dr. Debreceni Balázs
• 13. Urea cycle - Dr. Debreceni Balázs
• 14. Amino acid metabolism; the fate of the carbon skeleton - Dr. Debreceni Balázs
• 15. Synthesis of non-essential amino acids - Dr. Debreceni Balázs
• 16. Enzymopathies of amino acid metabolism - Dr. Debreceni Balázs
• 17. Synthesis of biologically active molecules from amino acids - Dr. Debreceni Balázs
• 18. Synthesis of purine and pyrimidine nucleotides - Marquettené Dr. Bock Ildikó
• 19. Degradation of purine and pyrimidine nucleotides - Marquettené Dr. Bock Ildikó
• 20. Genes and chromosomes - Dr. Veres Balázs
• 21. DNA replication - Dr. Veres Balázs
• 22. DNA repair - Dr. Veres Balázs
• 23. RNA metabolism 1 - Dr. Veres Balázs
• 24. RNA metabolism 2 - Dr. Veres Balázs
• 25. Short RNA molecules - Dr. Veres Balázs
• 26. Retroviruses - Dr. Veres Balázs
• 27. Protein synthesis 1. Participants and mechanism - Dr. Berente Zoltán
• 28. Protein synthesis 2. Regulation, posttranslational modifications - Dr. Berente Zoltán
• 29. Protein folding, chaperones - Dr. Berente Zoltán
• 30. Protein targeting and vesicular transport of proteins - Dr. Berente Zoltán
• 31. Intracellular proteolysis - Dr. Sümegi Balázs
• 32. Regulation of gene expression 1 - Dr. Veres Balázs
• 33. Regulation of gene expression 2 - Dr. Veres Balázs
• 34. Mitochondrial protein synthesis, mitochondrial genome - Dr. Veres Balázs
• 35. Recombinant DNA technologies - Dr. Debreceni Balázs
• 36. Hormones 1 - Dr. Tapodi Antal
• 37. Hormones 2 - Dr. Tapodi Antal
• 38. Cell signalling and kinases I - Dr. Veres Balázs
• 39. Cell signalling and kinases II - Dr. Veres Balázs
• 40. Cell signalling, CO and NO as signals, transcription factors - Dr. Veres Balázs
• 41. Water soluble vitamins - Marquettené Dr. Bock Ildikó
• 42. Lipid soluble vitamins - Marquettené Dr. Bock Ildikó

Practices

• 1. Preparation of buffer solutions
• 2. Preparation of buffer solutions
• 3. Basic biochemical laboratory techniques
• 4. Basic biochemical laboratory techniques
• 5. Inorganic phosphate requirement of glycolysis
• 6. Inorganic phosphate requirement of glycolysis
• 7. Concerted regulation of carbohydrate metabolism
• 8. Concerted regulation of carbohydrate metabolism
• 9. Exploration of the contents of a cell, subcellular fractionation
• 10. Exploration of the contents of a cell, subcellular fractionation
• 11. Concerted regulation of lipid metabolism
• 12. Concerted regulation of lipid metabolism
• 13. Determination of protein concentration
• 14. Determination of protein concentration
• 15. Mitochondrial oxidation; Clark electrode
• 16. Mitochondrial oxidation; Clark electrode
• 17. Affinity chromatography; Study of Complex I and III
• 18. Affinity chromatography; Study of Complex I and III
• 19. Inborn Errors of Metabolism I
• 20. Inborn Errors of Metabolism I
• 21. Student presentations
• 22. Student presentations
• 23. Inborn Errors of Metabolism II
• 24. Inborn Errors of Metabolism II
• 25. Study of chaperone function
• 26. Study of chaperone function
• 27. Consultation, student presentations
• 28. Consultation, student presentations

Seminars

Reading material

Obligatory literature

Nelson, Cox: Lehninger Principles of Biochemistry, 6th edition, W.H. Freeman, 2013.

Literature developed by the Department

http://aok.pte.hu/en/egyseg/oktatasianyagok/20

Notes

http://aok.pte.hu/en/egyseg/oktatasianyagok/20

Recommended literature

Berg, Tymoczko, Stryer: Biochemistry, 7th edition, W.H. Freeman, 2012.
Devlin: Textbook of Biochemistry with Clinical Correlations, 7th edition, Wiley, 2010.

Conditions for acceptance of the semester

Requirements of the acceptance of Biochemistry course are the following:
- No more than three absences from the laboratory practices
- Submission of at least 10 out of 12 short tests held in the first 10 minutes of the practices
- Achieving satisfactory level in at least seven of the 12 short tests

Mid-term exams

http://aok.pte.hu/en/egyseg/oktatasianyagok/20

Making up for missed classes

There is no possibility to make up the practices.

Exam topics/questions

The exams, including the retaken exams, are written exams of 27 open questions. Failure to answer more than two of the first 7 questions (less than 20 points out of 28) will automatically result in "failed" grade. The final mark comprises the results of the pre-lab test points (max. 12 p), and the 3 extra points as well which can be collected by the oral presentations.

Examiners

• Andreidesz Kitti
• Dr. Agócs Attila
• Dr. Berente Zoltán
• Dr. Bognár Zita
• Dr. Fekete Katalin
• Dr. Jávor-Hocsák Enikő
• Dr. Radnai Balázs
• Dr. Szabó Aliz
• Dr. Takátsy Anikó
• Dr. Tapodi Antal
• Dr. Veres Balázs
• Kovács Dominika
• Kőszegi Balázs
• Nagyné Dr. Kiss Gyöngyi

Instructor / tutor of practices and seminars

• Andreidesz Kitti
• Dr. Bognár Zita
• Dr. Fekete Katalin
• Dr. Jávor-Hocsák Enikő
• Dr. Takátsy Anikó
• Dr. Tapodi Antal
• Dr. Veres Balázs
• Kőszegi Balázs
• Nagyné Dr. Kiss Gyöngyi