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Education

Biochemistry - Practice

Data

Official data in SubjectManager for the following academic year: 2022-2023

Course director

Number of hours/semester

lectures: 0 hours

practices: 28 hours

seminars: 0 hours

total of: 28 hours

Subject data

  • Code of subject: OBA-108-G
  • 2 kredit
  • Biotechnology MSc
  • Basic modul
  • autumn
Prerequisites:

OBA-108-E parallel

Course headcount limitations

min. 4 – max. 20

Available as Campus course for . Campus-karok: ÁOK

Topic

The course helps to evolve good practical skills. It gives an overview on the techniques that are used in the basic biochemical-, biomedical research, both in the industrial area as well as in clinical research. The main focus is on solving of different scientific problems and through that a solution orientated education.

Lectures

Practices

  • 1. Solutions
  • 2. Solutions
  • 3. Preparation of buffer solution
  • 4. Preparation of buffer solution
  • 5. Protein function
  • 6. Basics of enzyme catalysis
  • 7. Measuring the proteint contant of different solutions
  • 8. Biuret reaction
  • 9. Oral presentations
  • 10. Oral presentations
  • 11. Enzyme activity measurement
  • 12. Inorganic phosphate demand of glycolysis
  • 13. Oral presentations
  • 14. Oral presentations
  • 15. Molecular recognition
  • 16. Affinity chromatography
  • 17. Apoptosis, necrosis
  • 18. Techniques to describe apoptotical processes or necrosis
  • 19. PAGE, Western blot
  • 20. PAGE, Western blot
  • 21. Antibody-Based techniques in biochemistry
  • 22. Antibody-Based techniques in biochemistry
  • 23. Working with cells
  • 24. Working with cells
  • 25. Bioanalytical teachniques
  • 26. Bioanalytical teachniques
  • 27. Consultation
  • 28. Exam

Seminars

Reading material

Obligatory literature

Albert L. Lehninger - David L. Nelson - Michael M.Cox: Principles of Biochemistry, Worth Publichers, Inc.

Literature developed by the Department

http://aok.pte.hu/hu/egyseg/oktatasianyagok/20

Notes

http://aok.pte.hu/hu/egyseg/oktatasianyagok/20

Recommended literature

Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr., Lubert Stryer: Biochemistry
Thomas M. Devlin: Textbook of Biochemistry. With Clinical Correlations, John Wiley & Sons, New York

Conditions for acceptance of the semester

Maximum of 15 % absence allowed

Mid-term exams

Oral presentations should be kept by every student on the 5th or 7th week from an optional topic, in the from of a ppt presentation. The theme of the presentation should have a biochemical connection, and should be adjusted with the subject leader.

Making up for missed classes

There is no possibility to retake the practice.

Exam topics/questions

A written exam that screens the basic methodological knowledge, and the capability of solving scientific problems.

Examiners

Instructor / tutor of practices and seminars

  • Dr. Juhász Kata
  • Dr. Takátsy Anikó
  • Nagyné Dr. Kiss Gyöngyi
Rólunk