Data
Official data in SubjectManager for the following academic year: 2019-2020
Course director
-
Dr. Hajnalka Gabriella ÁBRAHÁM
associate professor,
Department of Medical Biology and Central Electron Microscope Laboratory -
Number of hours/semester
lectures: 24 hours
practices: 0 hours
seminars: 0 hours
total of: 24 hours
Subject data
- Code of subject: OSF-EMA-T
- 2 kredit
- Dentistry
- Optional modul
- spring
-
Exam course:no
Course headcount limitations
min. 5 – max. 25
Topic
Methods of electron microscopic fixation, embedding and sectioning and the use of the electron microscope. Demonstration of the subcellular elements in details and of a few tissues and organs. Demonstration of the electron microscopic methods used in the biological research. The use of electron microscopic methods in the clinical practice.
The basic principles of electron microscopy will be discussed and we provide information about the mode and the use of electron microscopy in the basic research and in the clinical practice.
Lectures
- 1. Purpose and indication of the electron microscopic examination. The role of fixation in tissue preservation, the recognition of cells, cellular organelles. - Dr. Rimayné Dr. Ábrahám Hajnalka Gabriella
- 2. Most frequent failures in the electronmicroscopic practice - Dr. Seress László Antal
- 3. Optimal fixation for electron microscopy. Composition of fixatives for different tissue samples. - Dr. Seress László Antal
- 4. Samples taken by autopsy or biopsy. Perfusion of experimental animals for electron microscopy. Electron microscopy of formaldehyde fixed and paraffin embedded materials. - Dr. Seress László Antal
- 5. The ultrastructure of the cell I - Dr. Seress László Antal
- 6. The ultrastructure of the cell II - Dr. Seress László Antal
- 7. The ultrastructure of the cell III - Dr. Seress László Antal
- 8. The ultrastructure of the cell IV - Dr. Seress László Antal
- 9. The ultrastructure of the cell V - Dr. Rimayné Dr. Ábrahám Hajnalka Gabriella
- 10. The ultrastructure of the cells VI. Test. - Dr. Rimayné Dr. Ábrahám Hajnalka Gabriella
- 11. Ultrastructures of neurons (axon, dendrite, synapses) - Dr. Seress László Antal
- 12. Ultrastructure of glial cells - Dr. Seress László Antal
- 13. The ultrastructure of kidney - Dr. Rimayné Dr. Ábrahám Hajnalka Gabriella
- 14. The ultrastructure of the liver - Dr. Rimayné Dr. Ábrahám Hajnalka Gabriella
- 15. Ultrastructure of the muscle. - Dr. Rimayné Dr. Ábrahám Hajnalka Gabriella
- 16. Necrosis and apoptosis. - Dr. Rimayné Dr. Ábrahám Hajnalka Gabriella
- 17. Combined light and electron microscopic methods, such as Golgi/EM, Timm/EM, immunocytochemistry/EM. - Dr. Seress László Antal
- 18. Combination of degeneration and axon transport methods with electron microscopy. - Dr. Seress László Antal
- 19. Ultrastructure of bacteria and viruses. - Dr. Rimayné Dr. Ábrahám Hajnalka Gabriella
- 20. Cytoskeleton - Dr. Seress László Antal
- 21. Intercellular connections - Dr. Seress László Antal
- 22. The use of EM in biological research I - Dr. Seress László Antal
- 23. The use of EM in biological research II - Dr. Seress László Antal
- 24. Exam - Dr. Seress László Antal
Practices
Seminars
Reading material
Obligatory literature
Literature developed by the Department
Notes
Recommended literature
Suggested only:
Dr. Szabolcs Virágh: Ultrastructural Pathology and Diagnostic Electron Microscopy
Leon Weiss: Histology. Cell and Tissue Biology
Conditions for acceptance of the semester
Participation on 80% of the classes. Oral examination with analysis of 3 electron microscopic photographs
Mid-term exams
No possibility
Making up for missed classes
No possibility
Exam topics/questions
Exam topics are the same as lecture topics