Methods in Molecular Biology and Their Applications in Medical Practice

Data

Official data in SubjectManager for the following academic year: 2019-2020

Course director

  • Dr. Marianna PAP

    associate professor,
    Department of Medical Biology and Central Electron Microscope Laboratory

Number of hours/semester

lectures: 24 hours

practices: 0 hours

seminars: 0 hours

total of: 24 hours

Subject data

  • Code of subject: OXFMMO-z-T
  • 2 kredit
  • Dentistry
  • Optional modul
  • spring
Prerequisites:

OZAMF1-T completed

Course headcount limitations

min. 1

Topic

The aim of the course is to describe interesting and novel molecular biology technologies, including three-dimensional tissue culturing, bioprinting, new generation sequencing techniques, targeted genome editing, genetically modified organisms. We emphasize the importance and applicability of these methods in research, in the modeling and diagnosis of different diseases. We also describe the importance and application of artificial intelligence in future medicine.

Lectures

  • 1. Types of cell cultures - Dr. Pap Marianna
  • 2. Maintenance and manipulation of cell cultures - Dr. Pap Marianna
  • 3. 3D tissue culturing - Dr. Pap Marianna
  • 4. 3D tissue culturing - Dr. Pap Marianna
  • 5. Bioprinting - Dr. Pap Marianna
  • 6. Bioprinting - Dr. Pap Marianna
  • 7. Hybridization techniques - Dr. Pap Marianna
  • 8. PCR, RT-PCR - Dr. Pap Marianna
  • 9. Next generation sequencing techniques - Dr. Pap Marianna
  • 10. Next generation sequencing techniques - Dr. Pap Marianna
  • 11. Analysis of DNA methylation, epigenetics - Dr. Rauch Tibor Attila
  • 12. Analysis of DNA methylation, epigenetics - Dr. Rauch Tibor Attila
  • 13. Cloning, protein expression systems and their medical applications - Dr. Pap Marianna
  • 14. Cloning, protein expression systems and their medical applications - Dr. Pap Marianna
  • 15. Genome-editing technologies, medical applications and ethical concerns - Dr. Pap Marianna
  • 16. Genome-editing technologies, medical applications and ethical concerns - Dr. Pap Marianna
  • 17. Analysis of protein interactions - Dr. Pap Marianna
  • 18. Analysis of protein interactions - Dr. Pap Marianna
  • 19. Genetically-modified organisms and their applications in medicine - Dr. Pap Marianna
  • 20. Genetically-modified organisms and their applications in medicine - Dr. Pap Marianna
  • 21. Importance and application of artificial intelligence in medicine - Dr. Pap Marianna
  • 22. Importance and application of artificial intelligence in medicine - Dr. Pap Marianna
  • 23. Exam - Dr. Pap Marianna
  • 24. Exam - Dr. Pap Marianna

Practices

Seminars

Reading material

Obligatory literature

Literature developed by the Department

Lecture slides

Notes

Recommended literature

Conditions for acceptance of the semester

Maximum of 25 % absence allowed

Mid-term exams

Written test on the last week.

Making up for missed classes

No possibility to make up the missing lectures.

Exam topics/questions

.

Examiners

Instructor / tutor of practices and seminars