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Education

Methods in Molecular Biology and Their Applications in Medical Practice

Data

Official data in SubjectManager for the following academic year: 2022-2023

Course director

  • Dr. Marianna PAP

    associate professor,
    Department of Medical Biology and Central Electron Microscope Laboratory

Number of hours/semester

lectures: 24 hours

practices: 0 hours

seminars: 0 hours

total of: 24 hours

Subject data

  • Code of subject: OBF-085-T
  • 2 kredit
  • Biotechnology MSc
  • Optional modul
  • spring
Prerequisites:

-

Course headcount limitations

min. 1 – max. 200

Topic

The aim of the course is to describe interesting and novel molecular biology technologies, including three-dimensional tissue culturing, bioprinting, new generation sequencing techniques, human genetic markers, targeted genome editing. We emphasize the importance and applicability of these methods in research, in the modeling and diagnosis of different diseases.

Lectures

  • 1. Types of cell cultures - Dr. Pap Marianna
  • 2. Maintenance and manipulation of cell cultures - Dr. Pap Marianna
  • 3. 3D tissue culturing - general features - Dr. Pap Marianna
  • 4. 3D tissue culturing - cells and biomaterials - Dr. Pap Marianna
  • 5. Bioprinting - general features - Dr. Pap Marianna
  • 6. Bioprinting - medical application - Dr. Pap Marianna
  • 7. Types of PCR - Dr. Pap Marianna
  • 8. PCR - medical application - Dr. Pap Marianna
  • 9. Next generation sequencing techniques - Dr. Pap Marianna
  • 10. Next generation sequencing techniques - Dr. Pap Marianna
  • 11. Human genetic markers - general features - Dr. Pap Marianna
  • 12. Methods to study human genetic markers - Dr. Pap Marianna
  • 13. Genome editing - sequence-specific nucleases - Dr. Pap Marianna
  • 14. Genome editing - CRISPR/Cas9 system and medical application - Dr. Pap Marianna
  • 15. Epigenetics - Dr. Pap Marianna
  • 16. Methods to study DNA methylation - Dr. Pap Marianna
  • 17. Functional genomics - methods to study transcription - Dr. Pap Marianna
  • 18. Functional genomics - methods to study translation - Dr. Pap Marianna
  • 19. Molecular diagnostics - Dr. Pap Marianna
  • 20. Molecular diagnostics - Dr. Pap Marianna
  • 21. Types of gene therapy - Dr. Pap Marianna
  • 22. Gene therapy - medical application - Dr. Pap Marianna
  • 23. Exam - Dr. Pap Marianna
  • 24. Exam - Dr. Pap Marianna

Practices

Seminars

Reading material

Obligatory literature

Literature developed by the Department

Lecture slides

Notes

Recommended literature

Conditions for acceptance of the semester

Maximum of 25 % absence allowed

Mid-term exams

Written test on the last week.

Making up for missed classes

No possibility to make up the missing lectures.

Exam topics/questions

.

Examiners

Instructor / tutor of practices and seminars

Rólunk