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Medicine in the Future: Genetic Engineering and Biotechnology (Recombinant DNA/RNA/Protein Technologies)

Data

Official data in SubjectManager for the following academic year: 2022-2023

Course director

  • Rauch Tibor Attila

    senior research fellow,
    Department of Medical Biology and Central Electron Microscope Laboratory

Number of hours/semester

lectures: 16 hours

practices: 8 hours

seminars: 0 hours

total of: 24 hours

Subject data

  • Code of subject: OTF-GBJ-T
  • 2 kredit
  • Biotechnology BSc
  • Optional modul
  • autumn
Prerequisites:

OTV-SEBI-T completed , OTV-BIC1-T completed

Course headcount limitations

min. 3 – max. 15

Available as Campus course for 15 fő számára. Campus-karok: ÁOK ETK GYTK TTK

Topic

Genetic engineering techniques offer unprecedented therapeutic possibilities that will certainly play a central role in future medicine. Today's oncotherapy and rheumatology toolkits already rely to a large extent on genetically engineered protein and peptide-based drugs. During the COVID-19 epidemic, recombinant viral and RNA vaccines proved their efficacy. In the light of these facts, future scientists and clinicians will benefit from this course, which will provide them a theoretical background of genetic engineering and practical insights into basic recombinant DNA, RNA and protein expression technologies. Current legal and medical positions on the subject will also be discussed.

Lectures

  • 1. The subject and history of genetic engineering - Dr. Rauch Tibor Attila
  • 2. Ethical issues in genetic engineering - Dr. Rauch Tibor Attila
  • 3. DNA, RNA and protein extraction from medical samples: past and present - Dr. Rauch Tibor Attila
  • 4. Enzymes used in genetic engineering - Dr. Rauch Tibor Attila
  • 5. Basics of molecular cloning - Dr. Rauch Tibor Attila
  • 6. New genetic engineering technologies - Dr. Rauch Tibor Attila
  • 7. Protein overproduction in prokaryotic and eukaryotic systems - Dr. Rauch Tibor Attila
  • 8. Peptide- and protein-based therapies - Dr. Rauch Tibor Attila
  • 9. Classical approach of antibody production - Dr. Rauch Tibor Attila
  • 10. Production of antibodies with new technologies - Dr. Rauch Tibor Attila
  • 11. Bispecific antibodies - Dr. Rauch Tibor Attila
  • 12. CAR-T technology - Dr. Rauch Tibor Attila
  • 13. Classical vaccine production - Dr. Rauch Tibor Attila
  • 14. Recombinant viral vaccines - Dr. Rauch Tibor Attila
  • 15. Recombinant RNA vaccines - Dr. Rauch Tibor Attila
  • 16. Recombinant RNA- and DNA-based oncotherapy - Dr. Rauch Tibor Attila

Practices

  • 1. RNA and DNA isolation from blood, PCR primer design
  • 2. cDNA synthesis, PCR amplification
  • 3. Cloning into protein expression vector
  • 4. Recombinant protein overproduction and purification
  • 5. Biochemical analysis of purified protein
  • 6. Physiological testing of purified protein
  • 7. Oral presentation of a chosen topic
  • 8. Oral presentation of a chosen topic

Seminars

Reading material

Obligatory literature

Literature developed by the Department

PowerPoint slides of the course

Notes

Recommended literature

Conditions for acceptance of the semester

Maximum of 15 % absence allowed

Mid-term exams

N/A

Making up for missed classes

N/A

Exam topics/questions

1 The subject and history of genetic engineering
2 Ethical issues in genetic engineering
3 DNA, RNA and protein extraction from medical samples: past and present
4 Enzymes used in genetic engineering
5 Basics of molecular cloning
6 New genetic engineering technologies
7 Peptide- and protein-based therapies
8 Protein overproduction in prokaryotic and eukaryotic systems
9 Classical approach of antibody production
10 Production of antibodies with new technologies
11 Bispecific antibodies
12 CAR-T technology
13 Classical vaccine production
14 Recombinant viral vaccines
15 Recombinant RNA vaccines
16 Recombinant RNA- and DNA-based oncotherapy

Examiners

Instructor / tutor of practices and seminars

  • Dr. Rauch Tibor Attila
Rólunk