Molecular Biology and Bioinformatic Techniques from a Practical Approach

Data

Official data in SubjectManager for the following academic year: 2021-2022

Course director

Number of hours/semester

lectures: 12 hours

practices: 0 hours

seminars: 0 hours

total of: 12 hours

Subject data

  • Code of subject: OBF-BTP-T
  • 1 kredit
  • Biotechnology MSc
  • Optional modul
  • autumn
Prerequisites:

-

Course headcount limitations

min. 5 – max. 25

Available as Campus course for 1 fő számára. Campus-karok: ÁOK GYTK TTK

Topic

The aim of the course is to discuss the common DNA and genome editing techniques focusing on fluorescent protein tagging and bioinformatic tools used in genome and image analysis.

Lectures

  • 1. Detection of specific nucleotide sequences - Dr. Kovács Tamás
  • 2. Gene activity measurement (qPCR) - Dr. Kovács Tamás
  • 3. Online nucleotide databases - Dr. Ernszt Dávid
  • 4. Primer design and qPCR analysis - Dr. Ernszt Dávid
  • 5. Molecular cloning - Dr. Kovács Tamás
  • 6. Viral transduction and transfection techniques - Dr. Kovács Tamás
  • 7. Plasmid restriction mapping - Dr. Ernszt Dávid
  • 8. In silico analysis - Dr. Ernszt Dávid
  • 9. Genome editing techniques - Dr. Kovács Tamás
  • 10. CRISPR Cas9 - Dr. Kovács Tamás
  • 11. Basics of data analysis - Dr. Ernszt Dávid
  • 12. Basics of image analysis - Dr. Ernszt Dávid

Practices

Seminars

Reading material

Obligatory literature

Literature developed by the Department

Topics of the lectures will be available in form of ppt files.

Notes

Recommended literature

Conditions for acceptance of the semester

Maximum of 25 % absence allowed

Mid-term exams

Oral report based on the topics of lectures at the end of the course. Missed oral report can be catch up in that week.

Making up for missed classes

None

Exam topics/questions

Oral report based on the topics of lectures.

Examiners

Instructor / tutor of practices and seminars