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Introduction to Genetic Engineering

Data

Official data in SubjectManager for the following academic year: 2022-2023

Course director

Number of hours/semester

lectures: 0 hours

practices: 0 hours

seminars: 22 hours

total of: 22 hours

Subject data

  • Code of subject: OAF-BGS-T
  • 2 kredit
  • General Medicine
  • Optional modul
  • spring
Prerequisites:

-

Course headcount limitations

min. 1 – max. 20

Available as Campus course for 10 fő számára. Campus-karok: ÁOK TTK

Topic

The aim of these seminars is to introduce genetic engineering techniques to medical students.

Lectures

Practices

Seminars

  • 1. Structure and function of nucleic acids
  • 2. Modified and artificial nucleic acids
  • 3. Oligonucleotide and gene synthesis
  • 4. Gel electrophoresis and sequencing
  • 5. Polymerases
  • 6. Polymerase chain reaction
  • 7. Enzymes to manipulate nucleic acids I.
  • 8. Enzymes to manipulate nucleic acids II.
  • 9. cDNA cloning, basic cloning vectors
  • 10. Expression vectors
  • 11. Transformation, transfection
  • 12. Viral vectors, transposone systems
  • 13. Transgenic and knockout animals
  • 14. Lox-P system, conditional KO animals
  • 15. RNA interference, knockdown techniques
  • 16. Artificial nucleases, genome engineering
  • 17. Optogenetics
  • 18. Artificial evolution techniques
  • 19. Gene therapy
  • 20. Genetic engineering in drug discovery
  • 21. Project evaluation I
  • 22. Project evaluation II

Reading material

Obligatory literature

None

Literature developed by the Department

Power point slides

Notes

None

Recommended literature

None

Conditions for acceptance of the semester

None

Mid-term exams

None

Making up for missed classes

None

Exam topics/questions

None

Examiners

Instructor / tutor of practices and seminars

  • Dr. Sándor Zoltán
Rólunk