Supervisor: FISI, Viktória
Co-supervisor: Dr. NAGY, Tamás
Cellular volume regulation is one of the key functions that define any living cells. It affects and interacts many physiological process, including membrane traffic, cell migration, cell proliferation, adaptation to stress and environmental challenges. Despite this, volume regulation is often overlooked process and there are still many questions to be answered, related to both it's regulatory mechanisms and it's effect on other cellular functions.
The shape of the cells and the amount and organization of intracellular water is regulated by proteins. Since translated proteins can’t change their amino-acid sequence, post-translational modifications play an improtant role in the dynamic regulation of water. Phosphorylation, ubiquitination, glycosylation, binding of smaller molecules (ATP, GTP, cAMP, lipids), bindig to ions can all change the configuration and the hydorophobic properties of the proteins.
Previously we showed that increased O-Glycosylation of proteins can alter the osmotic resistance and the intracellular water diffusibility of the cells. In this study, our aim is to clarify the exact mechanism by which O-Glycosylation inflences water regulation; whether is it a) changing the hydrophobicity, b) changing the function of some specific proteins involved in volume regulation (aquaporin e.g.) or c) influencing signaling pathways.