Biophysics - Lecture

Adatok

A Tantárgybejelentőben megadott hivatalos adatok az alábbi tanévre: 2020-2021

Tematika

The aim of the Biophysics curse is to introduce the students to the methods and applications, which are routinely used in both the medical and pharmaceutical biotechnology. The principles of the state-of the art approaches and instrumentations are covered by the topics. The course presents diverse spectroscopic techniques (absorption, fluorescence, infrared, Raman, EPR, NMR) imaging approaches (light and fluorescence microscopy, EM, super-resolution fluorescence microscopy, MRI), radioactive applications and calorimetric and fast kinetics techniques. The lectures discuss in details the physical basis and principles of each approach and the field of applications. The practicals are dedicated to extend the students’ knowledge and routine in the use of the different techniques. The practicals lay special emphasis on presenting not only the routine applications, but advanced uses of each technique. The limitations, as well as the artefacts that can be caused by improper experimental planning are highlighted.

Előadások

• 1. Introduction - Dr. Bugyi Beáta
• 2. Databases, resources. - Dr. Bugyi Beáta
• 3. Electromagnetic radiations. - Dr. Grama László
• 4. Quantummechanical model of atoms, quantum numbers. - Dr. Grama László
• 5. Energy levels of atoms and molecules. - Dr. Lukács András Szilárd
• 6. Laser. - Dr. Lukács András Szilárd
• 7. UV-VIS spectroscopy. Circular dichroism (CD) spectroscopy. - Dr. Huber Tamás
• 8. Infrared and Raman spectroscopy. - Dr. Bugyi Beáta
• 9. Fluorescence spectroscopy, fluorescence parameters. Förster resonance energy transfer (FRET). - Dr. Huberné Dr. Barkó Szilvia
• 10. Flow cytometry. Fluorescence activated cell sorting. - Dr. Huberné Dr. Barkó Szilvia
• 11. Electron paramagnetic resonance (EPR), nuclear magnetic resonance (NMR) spectroscopy. - Dr. Grama László
• 12. Magnetic resonance imaging (MRI). - Dr. Grama László
• 13. Basics of light microscopy and fluorescence microscopy. - Dr. Bugyi Beáta
• 14. Advanced fluorescence microscopy: confocal microscopy, fluorescence recovery after photobleaching (FRAP), multi-photon microscopy, FRET, FLIM, TIRFM, STED, SIM, single molecule localization microscopy. - Dr. Bugyi Beáta
• 15. Scanning probe microscopy, electron microscopy. - Dr. Bugyi Beáta
• 16. Image analysis. - Dr. Bugyi Beáta
• 17. X-ray diffraction. - Dr. Talián Csaba Gábor
• 18. Rapid kinetic methods: stopped-flow, surface plasmon resonance (SPR). - Dr. Kengyel András Miklós
• 19. Sound. Ultrasound. - Dr. Kengyel András Miklós
• 20. Radioactivity, interaction of radioactive radiations with matter. - Dr. Szabó-Meleg Edina
• 21. Dosimetry, detection of radioctive radiations. - Dr. Szabó-Meleg Edina
• 22. Biological effects of radioactive radiations. - Dr. Kengyel András Miklós
• 23. Gamma-camera, computed tomography (CT), single-photon emission computed tomography (SPECT), positron emission tomography (PET). - Dr. Kengyel András Miklós
• 24. Thermodynamics: laws, thermodynamic potentials. - Dr. Lukács András Szilárd
• 25. Calorimetric methods: differential scanning calorimetry (DSC), isothermal titration calorimetry (ITC). - Dr. Lukács András Szilárd
• 26. Analytical separation techniqes: sedimentation, electrophoresis, chromatography. - Dr. Talián Csaba Gábor
• 27. Mass spectrometry. - Dr. Talián Csaba Gábor
• 28. Revision - Dr. Bugyi Beáta

Gyakorlatok

Szemináriumok

A tananyag elsajátításához szükséges segédanyagok

Kötelező irodalom

Saját oktatási anyag

http://www.biofizika.aok.pte.hu/tantargy/Molecular_Biophysics_Lectures_-_Biotechnology

Jegyzet

http://www.biofizika.aok.pte.hu/tantargy/Molecular_Biophysics_Lectures_-_Biotechnology

Ajánlott irodalom

A félév elfogadásának feltételei

Maximum of 15 % absence allowed

Félévközi ellenőrzések

A kurzus oktatóival egyeztetve.

Távolmaradás pótlásának lehetőségei

A kurzus oktatóival egyeztetve.

Vizsgakérdések

http://www.biofizika.aok.pte.hu/tantargy/Molecular_Biophysics_Lectures_-_Biotechnology

Vizsgáztatók

• Dr. Bugyi Beáta
• Dr. Bukovics Péter

Gyakorlatok, szemináriumok oktatói

• Dr. Bugyi Beáta