Data
Official data in SubjectManager for the following academic year: 2023-2024
Course director
-
Dr. Anita BUFA
assistant professor,
Institute of Bioanalysis -
Number of hours/semester
lectures: 6 hours
practices: 6 hours
seminars: 0 hours
total of: 12 hours
Subject data
- Code of subject: OBF-081-T
- 1 kredit
- Biotechnology MSc
- Optional modul
- both semesters
-
Course headcount limitations
min. 5 – max. 10
Available as Campus course for 1 fő számára. Campus-karok: TTK
Topic
The participants are going to be familiarised theoretically and practically with:
- pre-treatment of urine sample
- qualitative and quantitative analysis of 24 androgen, progesterone and corticoid metabolites by gas chromatography using mass selective detector
- evaluation of urinary steroid profiles
Lectures
- 1. Urinary steroid metabolites - Dr. Bufa Anita
- 2. Pre-treatment of urine sample - Dr. Bufa Anita
- 3. Gas chromatography-Mass spectrometry - Dr. Bufa Anita
- 4. Qualitative analysis of urinary steroid metabolites - Dr. Bufa Anita
- 5. Quantitative analysis of urinary steroid metabolites - Dr. Bufa Anita
- 6. Evaluation of urinary steroid profile - Dr. Bufa Anita
Practices
- 1. Solid-phase extraction of urinary steroid metabolites
- 2. Enzymatic hydrolysis of urinary steroid metabolites
- 3. Derivatization of urinary steroid metabolites
- 4. Chromatographic cleaning
- 5. Determination of urinary steroid profiles by GC-MS technic
- 6. Qualitative and quantitative analysis of urinary steroid metabolites
Seminars
Reading material
Obligatory literature
Literature developed by the Department
It is going to be available on the website of Teams group and PotePedia.
Notes
Recommended literature
Conditions for acceptance of the semester
Maximum of 15 % absence allowed
Mid-term exams
After the course a written summary.
Making up for missed classes
Consultation with course director is required. The necessary information is going to be presented on the course.
Exam topics/questions
The necessary information is going to be presented on the course.
Examiners
Instructor / tutor of practices and seminars
- Dr. Bufa Anita