Biochemistry II

Data

Official data in SubjectManager for the following academic year: 2023-2024

Topic

Biochemistry II is built on the already known energy providing and biosynthetic pathways. We will study the metabolic regulations at different levels: cellular, tissues and whole organism. This means the most important information about enzymes and hormones: their synthesis and mechanisms of action. Especially enzymes are the subject of biotechnology: they are products as well as tools. Considering the human body, we discuss the energy supplies in fasting, sport, work, and also the most frequent pathobiochemical situations (diabetes mellitus). Special subject is the transmembrane processes, which are the targets of a large number of drugs.

Lectures

• 1. Biomembranes - Jánosa Gergely
• 2. Transport processes - Dr. Sipos Katalin
• 3. Transport processes - Dr. Sipos Katalin
• 4. Amino acids: synthesis and degradation - Dr. Farkas Viktória
• 5. Urea cycle - Dr. Sipos Katalin
• 6. Purine and pyrimidine synthesis - Dr. Farkas Viktória
• 7. Degradation of nucleotides - Dr. Farkas Viktória
• 8. Neurotransmitters - Pap Ramóna
• 9. Role of the liver in metabolism - Dr. Sipos Katalin
• 10. Development of drugs - Dr. Pandur Edina
• 11. Drugs in biotechnology - Dr. Pandur Edina
• 12. Metabolism of drugs - Dr. Sipos Katalin
• 13. Genetic diseases of metabolism - Pap Ramóna
• 14. Consultation - Dr. Sipos Katalin

Practices

• 1. General laboratory instructions. Making report of the practice.
• 2. General laboratory instructions. Making report of the practice.
• 3. Preparation of cell lysate. Acrylamide gel.
• 4. Preparation of cell lysate. Acrylamide gel.
• 5. Western blotting
• 6. Western blotting
• 7. Anorganic phosphate usage in fermentation
• 8. Anorganic phosphate usage in fermentation
• 9. Lactate dehydrogenase enzyme
• 10. Lactate dehydrogenase enzyme
• 11. Alkalic phosphatase. Inhibition of the enzyme
• 12. Alkalic phosphatase. Inhibition of the enzyme
• 13. Determination of blood sugar
• 14. Determination of blood sugar
• 15. Determination of cholesterol level
• 16. Determination of cholesterol level
• 17. Determination of uric acid level
• 18. Determination of uric acid level
• 19. HPLC
• 20. HPLC
• 21. Mass spectrometry
• 22. Mass spectrometry
• 23. Toxicology - determination of ethanol level in blood
• 24. Toxicology - determination of ethanol level in blood
• 25. Institute of Laboratory Medicine - human samples for diagnosis, automated systems in a laboratory
• 26. Institute of Laboratory Medicine - human samples for diagnosis, automated systems in a laboratory
• 27. Consultation
• 28. Consultation

Seminars

• 1. Biochemistry of vitamins
• 2. Digestion and absorption of macronutrients
• 3. Hormones
• 4. Diabetes mellitus
• 5. Muscle, brain, kidney, sport, pregnancy
• 6. Starvation, fed state
• 7. Metabolism of lipids
• 8. Carbohydrate metabolism
• 9. Regulation of glycolysis, gluconeogenesis
• 10. Regulation of citric acid cycle, ATP synthesis
• 11. Regulation of glycogen and cholesterol metabolism
• 12. Metabolism of alcohol
• 13. Consultation
• 14. Consultation

Reading material

Obligatory literature

Literature developed by the Department

The materials of the lectures and seminars will appear on Neptune.

Notes

Recommended literature

Ch. P. Woodbury: Biochemistry for the Pharmaceutical Sciences
Raymond S. Ochs: Biochemistry
Thomas M. Devlin: Textbook of Biochemistry with Clinical Correlations

Conditions for acceptance of the semester

Maximum of 25 % absence allowed

Mid-term exams

There is no written mid-term exam in the semester.

Making up for missed classes

According to personal agreement

Exam topics/questions

There are no given exam questions. The topics of the writtenexam will be the materials of lectures and seminars. There essay questions in the final exam about known topics.

Examiners

• Dr. Farkas Viktória
• Dr. Pandur Edina
• Dr. Sipos Katalin

Instructor / tutor of practices and seminars

• Dr. Farkas Viktória
• Dr. Horváth Adrienn
• Dr. Sipos Katalin
• Jánosa Gergely
• Pap Ramóna