Data
Official data in SubjectManager for the following academic year: 2023-2024
Course director
-
Dr. Anikó TAKÁTSY
associate professor,
Department of Biochemistry and Medical Chemistry -
Number of hours/semester
lectures: 0 hours
practices: 28 hours
seminars: 0 hours
total of: 28 hours
Subject data
- Code of subject: OBA-108-G
- 2 kredit
- Biotechnology MSc
- Basic modul
- autumn
OBA-108-E parallel
Course headcount limitations
min. 5 – max. 20
Available as Campus course for . Campus-karok: ÁOK
Topic
The course helps to evolve good practical skills. It gives an overview on the techniques that are used in the basic biochemical-, biomedical research, both in the industrial area as well as in clinical research. The main focus is on solving of different scientific problems and through that a solution orientated education.
Lectures
Practices
- 1. Solutions
- 2. Solutions
- 3. Preparation of buffer solution
- 4. Preparation of buffer solution
- 5. Protein function
- 6. Basics of enzyme catalysis
- 7. Measuring the proteint contant of different solutions
- 8. Biuret reaction
- 9. Oral presentations
- 10. Oral presentations
- 11. Enzyme activity measurement
- 12. Inorganic phosphate demand of glycolysis
- 13. Oral presentations
- 14. Oral presentations
- 15. Molecular recognition
- 16. Affinity chromatography
- 17. Apoptosis, necrosis
- 18. Techniques to describe apoptotical processes or necrosis
- 19. PAGE, Western blot
- 20. PAGE, Western blot
- 21. Antibody-Based techniques in biochemistry
- 22. Antibody-Based techniques in biochemistry
- 23. Working with cells
- 24. Working with cells
- 25. Bioanalytical teachniques
- 26. Bioanalytical teachniques
- 27. Consultation
- 28. Exam
Seminars
Reading material
Obligatory literature
Albert L. Lehninger - David L. Nelson - Michael M.Cox: Principles of Biochemistry, Worth Publichers, Inc.
Literature developed by the Department
Teams: OBA-108-G-2023241-MEA-A108-G-B1
http://aok.pte.hu/hu/egyseg/oktatasianyagok/20
Notes
Teams: OBA-108-G-2023241-MEA-A108-G-B1
http://aok.pte.hu/hu/egyseg/oktatasianyagok/20
Recommended literature
Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr., Lubert Stryer: Biochemistry
Thomas M. Devlin: Textbook of Biochemistry. With Clinical Correlations, John Wiley & Sons, New York
Conditions for acceptance of the semester
Maximum of 15 % absence allowed
Mid-term exams
Oral presentations should be kept by every student on the 5th or 7th week from an optional topic, in the from of a ppt presentation. The theme of the presentation should have a biochemical connection, and should be adjusted with the subject leader.
A written exam is going to be written in the 14th week of the semester that screens the basic methodological knowledge, and the capability of solving scientific problems. In case of unsuccesful exam, it can be retaken in the first two weeks of the exam periode.
Making up for missed classes
There is no possibility to retake the practice.
Exam topics/questions
Teams: OBA-108-G-2023241-MEA-A108-G-B1
Examiners
Instructor / tutor of practices and seminars
- Dr. Juhász Kata
- Dr. Takátsy Anikó
- Nagyné Dr. Kiss Gyöngyi