Microbiology 2 - Practice

Data

Official data in SubjectManager for the following academic year: 2023-2024

Topic

The objective is to provide a solid knowledge of microbiological diagnostic methods, sterility and pyrogenicity tests. By active participation in the practical, students will gain the skill of aseptic technique in microbiology and pharmacology.

Lectures

Practices

• 1. Introduction, safety regulations. The microscope, wet mount and stained preparations
• 2. Introduction, safety regulations. The microscope, wet mount and stained preparations
• 3. Cultivation of bacteria, media and techniques.
• 4. Cultivation of bacteria, media and techniques.
• 5. Identification of bacteria
• 6. Identification of bacteria
• 7. Serological methods in microbiology. Immunotherapy.
• 8. Serological methods in microbiology. Immunotherapy.
• 9. Molecular methods in clinical microbiology
• 10. Molecular methods in clinical microbiology
• 11. Sterilisation and disinfection, Sterility and pyrogen testing methods. Demonstration of the effect of disinfectants on skin flora
• 12. Sterilisation and disinfection, Sterility and pyrogen testing methods. Demonstration of the effect of disinfectants on skin flora
• 13. Antibiotic sensitivity (Kirby-Bauer method, MIC, MBC, E-test)
• 14. Antibiotic sensitivity (Kirby-Bauer method, MIC, MBC, E-test)
• 15. Antibiotic concentration in body fluids. Detection of enzymes and compounds which neutralize antibiotics. Interactions of antibiotics
• 16. Antibiotic concentration in body fluids. Detection of enzymes and compounds which neutralize antibiotics. Interactions of antibiotics
• 17. Diagnosis of bacterial wound infection. Sepsis - blood culture
• 18. Diagnosis of bacterial wound infection. Sepsis - blood culture
• 19. Bacterial diagnosis of gastrointestinal infections
• 20. Bacterial diagnosis of gastrointestinal infections
• 21. Bacterial diagnosis of urinary tract infections
• 22. Bacterial diagnosis of urinary tract infections
• 23. Bacterial diagnosis of respiratory tract infections
• 24. Bacterial diagnosis of respiratory tract infections
• 25. Microbiological diagnosis of fungal and parasitic infections
• 26. Microbiological diagnosis of fungal and parasitic infections
• 27. Methods used in diagnostic virology
• 28. Methods used in diagnostic virology

Seminars

Reading material

Obligatory literature

Denyer SP ,Hodges NA, Gorman SP, Gilmore BF: Hugo and Russells Pharmaceutical Microbiology, 8th Edition, Blackwell Science Ltd
2018. ISBN: 978-1-119-43449-8
Tim Sandle: Pharmaceutical Microbiology : Essentials for Quality Assurance and Quality Control, ELSEVIER SCIENCE & TECHNOLOGY ,
Woodhead Publishing Ltd, 2015 ISBN10 0081000227

Literature developed by the Department

-

Notes

Practices' materials on the Teams

Recommended literature

Dr. Patrick R. Murray, Dr. Ken S. Rosenthal and Dr. Michael A. Pfaller (eds.): Medical Microbiology, 9th edition, Elsevier Saunders 2020 ISBN13 (EAN): 9780323673228

Conditions for acceptance of the semester

Maximum of 15 % absence allowed

Mid-term exams

End of the course the students will take a lab practice exam.

Making up for missed classes

Consultation with supervisor

Exam topics/questions

Exam questions are based on the topic of the practicals.

Examiners

Instructor / tutor of practices and seminars

• Dr. Bátai Istvánné (Dr. Kerényi Monika)
• Dr. Kocsis Béla
• Dr. Mestyán Gyula
• Dr. Schneider György József
• Dr. Szereday László
• Dr. Tigyi Zoltán
• Zsoldiné Dr. Urbán Edit