Biomedical Microbiology

Data

Official data in SubjectManager for the following academic year: 2023-2024

Topic

The course briefly describes the features of the human microbiota, microflora and pathogens and their interactions with the human body. It will discuss the medical/pharmaceutical role of microbial products and the impact of microbiology on the development of different industrial technologies such as pharmaceutical, medical diagnostic and food technologies. Lectures and practicals will provide an overview of the latest technologies, their theoretical background and their applicability in different industrial fields.
The practicals are aimed to familiarise the students with the basic techniques and methods of microbiology by carrying out tasks independently. Students will be familiarised with methods of sterilisation, disinfection and sanitisation, aseptic laboratory work, the use techniques of different microbiological cultures and different detection and identification methods of microbes. Genetic engineering methods used in the field of microbiology will also be introduced.

Lectures

• 1. Human Microbiotas and ineraction with human body - Dr. Polgár Beáta
• 2. Bacterial strategies (virulence factors, pathogenicity) in human body - Dr. Schneider György József
• 3. Aerobic gram-pozitive bacteria causing human infections - Dr. Bátai Istvánné (Dr. Kerényi Monika)
• 4. Anaerobic gram-pozitive bacteria causing human infections - Zsoldiné Dr. Urbán Edit
• 5. Gram- negative bacteria causing infection - Dr. Schneider György József
• 6. Non-fermenters in human infection - Dr. Bátai Istvánné (Dr. Kerényi Monika)
• 7. Anerobic gram-negative bacteria - Zsoldiné Dr. Urbán Edit
• 8. Spirochetes, obligate intracellular pathogens - Dr. Bátai Istvánné (Dr. Kerényi Monika)
• 9. Mycobacteria - Dr. Lutz Zsolt
• 10. Fungal pathogens - Zsoldiné Dr. Urbán Edit
• 11. Parazites - Dr. Palkovics Tamás
• 12. Viral infection - Dr. Pankovics Péter
• 13. Microbial products in the medicine and pharmaceutical industry, vaccine developments - Dr. Polgár Beáta
• 14. Antimicrobial drugs - Zsoldiné Dr. Urbán Edit

Practices

• 1. Preparation of different liquid and solid media
• 2. Preparation of different liquid and solid media
• 3. Microbiological methods in diagnostic laboratory, preparation and staining of smear , microscopy
• 4. Staining methods Gram's staining, simple staining, acid-fast staining, capsule staining
• 5. Inoculation methods from different specimen
• 6. Culture on different media
• 7. Identification of bacterial colonies (biochemical test)
• 8. Other identification methods (e.g. MALDI-Tof MS)
• 9. Serologic methods in the bacterial identification
• 10. Molecular methods in the bacterial identification
• 11. Antibiotic susceptibility tests (Disc diffusion test, microdilutions and agar dilution methods)
• 12. Evaluation of antibiotic susceptible tsets results
• 13. Culture media used for fungus, fungal culture
• 14. Lab diagnosis of fungal infection
• 15. Molecular methods in bacteriology
• 16. Molecular methods in virology
• 17. Serological methods in virology
• 18. Serological methods in parazitology
• 19. Stool specimen examination in parazitology
• 20. Other specimen for parazitological investigation
• 21. Semi-analyser and automatisation
• 22. Sterility and pyrogenic investigation of the pharmacetical products
• 23. Epidemiological investigation - plazmid preparation
• 24. Epidemiological investigation - PFGE, MLST
• 25. Bacteriophage isolation
• 26. Bacteriophage using as vector and control of biofilm
• 27. Varios experiments
• 28. Evaluation of the microbiological experiments

Seminars

Reading material

Obligatory literature

Ford, Michael: Medical Microbiology (Fundamentals of Biomedical Science), ISBN-13: 978-0198818144, ISBN-10: 0198818149
Lee Yuan Kun ed. Microbial Biotechnology: Principles and Applications (3rd Edition ) 2013 ISBN-13: 978-9814366823 ISBN-10: 981436682X

Literature developed by the Department

lecture notes on the teams

Notes

Recommended literature

Madigan, Michael T.; Bender, Kelly S.; Buckley, Daniel H.; Sattley, W. Matthew; Stahl, David A.: Brock Biology of Microorganisms (16th Edition) 2020, Person ISBN 10: 0134874404 ISBN 13:9780134874401

Conditions for acceptance of the semester

Maximum of 15 % absence allowed

Mid-term exams

Completed assessment in laboratory practice is required

Making up for missed classes

Consultation with the supervisor

Exam topics/questions

Topics of the laboratory practices

Examiners

• Dr. Bátai Istvánné (Dr. Kerényi Monika)
• Dr. Polgár Beáta
• Dr. Schneider György József
• Zsoldiné Dr. Urbán Edit

Instructor / tutor of practices and seminars

• Dr. Bátai Istvánné (Dr. Kerényi Monika)
• Dr. Kocsis Béla
• Dr. Lutz Zsolt
• Dr. Palkovics Tamás
• Dr. Pankovics Péter
• Dr. Polgár Beáta
• Dr. Schneider György József
• Dr. Szereday László
• Zsoldiné Dr. Urbán Edit