Data
Official data in SubjectManager for the following academic year: 2023-2024
Course director
-
Dr. Tibor Attila RAUCH
senior research fellow,
Department of Medical Biology and Central Electron Microscope Laboratory -
Number of hours/semester
lectures: 16 hours
practices: 8 hours
seminars: 0 hours
total of: 24 hours
Subject data
- Code of subject: OTF-GBJ-T
- 2 kredit
- Biotechnology BSc
- Optional modul
- autumn
OTV-SEBI-T completed , OTV-BIC1-T completed
Course headcount limitations
min. 5 – max. 15
Available as Campus course for 15 fő számára. Campus-karok: ÁOK ETK GYTK TTK
Topic
Genetic engineering techniques offer unprecedented therapeutic possibilities that will certainly play a central role in future medicine. Today's oncotherapy and rheumatology toolkits already rely to a large extent on genetically engineered protein and peptide-based drugs. During the COVID-19 epidemic, recombinant viral and RNA vaccines proved their efficacy. In the light of these facts, future scientists and clinicians will benefit from this course, which will provide them a theoretical background of genetic engineering and practical insights into basic recombinant DNA, RNA and protein expression technologies. Current legal and medical positions on the subject will also be discussed.
Lectures
- 1. The subject and history of genetic engineering - Dr. Rauch Tibor Attila
- 2. Ethical issues in genetic engineering - Dr. Rauch Tibor Attila
- 3. DNA, RNA and protein extraction from medical samples: past and present - Dr. Rauch Tibor Attila
- 4. Enzymes used in genetic engineering - Dr. Rauch Tibor Attila
- 5. Basics of molecular cloning - Dr. Rauch Tibor Attila
- 6. New genetic engineering technologies - Dr. Rauch Tibor Attila
- 7. Protein overproduction in prokaryotic and eukaryotic systems - Dr. Rauch Tibor Attila
- 8. Peptide- and protein-based therapies - Dr. Rauch Tibor Attila
- 9. Classical approach of antibody production - Dr. Rauch Tibor Attila
- 10. Production of antibodies with new technologies - Dr. Rauch Tibor Attila
- 11. Bispecific antibodies - Dr. Rauch Tibor Attila
- 12. CAR-T technology - Dr. Rauch Tibor Attila
- 13. Classical vaccine production - Dr. Rauch Tibor Attila
- 14. Recombinant viral vaccines - Dr. Rauch Tibor Attila
- 15. Recombinant RNA vaccines - Dr. Rauch Tibor Attila
- 16. Recombinant RNA- and DNA-based oncotherapy - Dr. Rauch Tibor Attila
Practices
- 1. RNA and DNA isolation from blood, PCR primer design
- 2. cDNA synthesis, PCR amplification
- 3. Cloning into protein expression vector
- 4. Recombinant protein overproduction and purification
- 5. Biochemical analysis of purified protein
- 6. Physiological testing of purified protein
- 7. Oral presentation of a chosen topic
- 8. Oral presentation of a chosen topic
Seminars
Reading material
Obligatory literature
Literature developed by the Department
PowerPoint slides of the course
Notes
Recommended literature
Conditions for acceptance of the semester
Maximum of 15 % absence allowed
Mid-term exams
N/A
Making up for missed classes
N/A
Exam topics/questions
1 The subject and history of genetic engineering
2 Ethical issues in genetic engineering
3 DNA, RNA and protein extraction from medical samples: past and present
4 Enzymes used in genetic engineering
5 Basics of molecular cloning
6 New genetic engineering technologies
7 Peptide- and protein-based therapies
8 Protein overproduction in prokaryotic and eukaryotic systems
9 Classical approach of antibody production
10 Production of antibodies with new technologies
11 Bispecific antibodies
12 CAR-T technology
13 Classical vaccine production
14 Recombinant viral vaccines
15 Recombinant RNA vaccines
16 Recombinant RNA- and DNA-based oncotherapy
Examiners
Instructor / tutor of practices and seminars
- Dr. Rauch Tibor Attila