Modern Biophysical Research Methods

Data

Official data in SubjectManager for the following academic year: 2025-2026

Course director

Number of hours/semester

lectures: 0 hours

practices: 12 hours

seminars: 12 hours

total of: 24 hours

Subject data

  • Code of subject: OXF-MBK-h-T
  • 2 kredit
  • Allgemeine Humanmedizin
  • Optional modul
  • autumn
Prerequisites:

ODA-BI2-T finished

Course headcount limitations

min. 5 – max. 25

Topic

The course addresses the physics and biophysics-based research methods employed in life sciences. The practicals will familiarize the students with the methods, including a hands-on experience with the instruments.

Lectures

Practices

  • 1. Absorption photometry - Hild Gábor
  • 2. Absorption photometry - Hild Gábor
  • 3. Fluorescence spectroscopy - Hild Gábor
  • 4. Fluorescence spectroscopy - Hild Gábor
  • 5. Transient kinetic methods - Hild Gábor
  • 6. Transient kinetic methods - Hild Gábor
  • 7. Flow cytometry - Hild Gábor
  • 8. Flow cytometry - Hild Gábor
  • 9. Fluorescence and confocal microscopy - Hild Gábor
  • 10. Fluorescence and confocal microscopy - Hild Gábor
  • 11. ESR and NMR spectroscopy - Hild Gábor
  • 12. Differential scanning calorimetry - Hild Gábor

Seminars

  • 1. Foundations of spectroscopic methods - Hild Gábor
  • 2. Absorption photometry - Hild Gábor
  • 3. Raman, infrared, CD, FTIR spectroscopy - Hild Gábor
  • 4. Fluorescence spectroscopy - Hild Gábor
  • 5. Fluorescence spectroscopic methods (FRET, quenching, anistropy) - Hild Gábor
  • 6. Transient kinetic methods - Hild Gábor
  • 7. Flow cytometry - Hild Gábor
  • 8. Fluorescence and confocal microscopy - Hild Gábor
  • 9. ESR and NMR spectroscopy - Hild Gábor
  • 10. Molecular structure determination by X-ray diffraction - Hild Gábor
  • 11. Atomic force microscopy. Differential scanning calorimetry - Hild Gábor
  • 12. Thermogravimetry. Combination of thermoanalytical methods with other techniques - Hild Gábor

Reading material

Obligatory literature

Literature developed by the Department

http://biofizika.aok.pte.hu

Notes

Recommended literature

Conditions for acceptance of the semester

Maximum of three absences either from the seminars or practicals.

Mid-term exams

-

Making up for missed classes

None.

Exam topics/questions

Can be found on the departmental website (http://biofizika.aok.pte.hu)

Examiners

Instructor / tutor of practices and seminars

  • Hild Gábor