Biophysics - Practice

Data

Official data in SubjectManager for the following academic year: 2022-2023

Topic

The Biophysics curse aims to introduce the students to the methods and applications, which are routinely used in both medical and pharmaceutical biotechnology. The principles of the state-of-the-art approaches and instrumentations are covered by the topics. The course presents diverse spectroscopic techniques (absorption, fluorescence, infrared, Raman, EPR, NMR) imaging approaches (light and fluorescence microscopy, EM, super-resolution fluorescence microscopy, MRI), radioactive applications and calorimetric and fast kinetics techniques. The lectures discuss in detail the physical basis and principles of each approach and the field of applications. The practicals are dedicated to extending the students' knowledge and routine in the use of the different techniques. The practicals lay special emphasis on presenting not only the routine applications but advanced uses of each technique. The limitations, as well as the artifacts that can be caused by improper experimental planning, are highlighted.

Lectures

Practices

• 1. Bioinformatic resources
• 2. Bioinformatic resources
• 3. Light microscopy and image analysis 1
• 4. Light microscopy and image analysis 1
• 5. Light microscopy and image analysis 2
• 6. Light microscopy and image analysis 2
• 7. Spectroscopy 1
• 8. Spectroscopy 1
• 9. Spectroscopy 2
• 10. Spectroscopy 2
• 11. Multicolor fluorescence
• 12. Multicolor fluorescence
• 13. Analytical techniques
• 14. Analytical techniques

Seminars

Reading material

Obligatory literature

Literature developed by the Department

Homepage of the Department of Biophysics:
https://aok.pte.hu/en/egyseg/10/oktatasi-anyagok

Notes

Recommended literature

Conditions for acceptance of the semester

Maximum of 15 % absence allowed

Mid-term exams

Two written tests are scheduled during the semester covering the topics/calculations/etc discussed during the practices. The expected dates: 7th week, 14th week. The practical grade is obtained based on the average of the two grades of the tests.

Grading policy:
< 60% fail (1)
60 – 69% satisfactory (2)
70 – 79% average (3)
80 – 89% good (4)
> 90% excellent (5)

To pass, at least 60% has to be reached at each test.
Two retake tests will be scheduled at the end of the semester/exam period. The schedule will be provided during the semester.

Making up for missed classes

The schedule for making up the missed practices will be provided during the semester.

Exam topics/questions

Topics of the exam questions:
Electromagnetic waves
Quantum numbers, NMR, MRI
Energy levels of atoms and molecules
Laser
UV-VIS absorption spectroscopy
Fluorescence spectroscopy
Infrared and Raman spectroscopy
Rapid kinetic methods
Flow cytometry
Light and fluorescence microscopy
Advanced fluorescence microscopy
Image analysis
Sound, ultrasound
Gamma camera, computed tomography (CT), single-photon emission computed tomography (SPECT), positron emission tomography (PET)
Radioactivity, the interaction of radioactive radiations with matter
Biological effects of radioactive radiations, dosimetry
X-ray diffraction
Thermodynamics: laws and thermodynamic potentials
Calorimetry: differential scanning calorimetry (DSC), isothermal titration calorimetry (ITC)
Analytical separation techniques: sedimentation, electrophoresis
Analytical separation techniques: chromatographic techniques
Mass spectrometry

Examiners

Instructor / tutor of practices and seminars

• Dr. Bugyi Beáta
• Dr. Bukovics Péter
• Dr. Huber Tamás
• Dr. Kengyel András Miklós
• Dr. Szabó-Meleg Edina
• Karádi Kristóf Kálmán
• Tempfliné Pirisi Katalin Erzsébet