Biophysics - Lecture

Data

Official data in SubjectManager for the following academic year: 2023-2024

Topic

The Biophysics curse aims to introduce the students to the methods and applications, which are routinely used in both medical and pharmaceutical biotechnology. The principles of the state-of-the-art approaches and instrumentations are covered by the topics. The course presents diverse spectroscopic techniques (absorption, fluorescence, infrared, Raman, EPR, NMR) imaging approaches (light and fluorescence microscopy, EM, super-resolution fluorescence microscopy, MRI), radioactive applications and calorimetric and fast kinetics techniques. The lectures discuss in detail the physical basis and principles of each approach and the field of applications. The practicals are dedicated to extending the students' knowledge and routine in the use of the different techniques. The practicals lay special emphasis on presenting not only the routine applications but advanced uses of each technique. The limitations, as well as the artifacts that can be caused by improper experimental planning, are highlighted.

Lectures

• 1. Introduction. Molecular biophysics in biotechnology. - Dr. Bugyi Beáta
• 2. Databases. Bioinformatics resources. - Dr. Bugyi Beáta
• 3. Electromagnetic waves, the electromagnetic spectrum. - Dr. Grama László
• 4. The wave and particle nature of light. - Dr. Grama László
• 5. Energy levels of atoms and molecules. - Dr. Lukács András Szilárd
• 6. Laser. - Dr. Lukács András Szilárd
• 7. UV-VIS spectroscopy. - Dr. Huber Tamás
• 8. Infrared and Raman spectroscopy. - Dr. Bugyi Beáta
• 9. Fluorescence spectroscopy. - Dr. Huberné Dr. Barkó Szilvia
• 10. Flow cytometry. Fluorescence activated cell sorting (FACS). - Dr. Huberné Dr. Barkó Szilvia
• 11. Multicolor fluorescence: spectral overlap, Förster resonance energy transfer (FRET), compensation. - Dr. Bugyi Beáta
• 12. Spin. Electron paramagnetic resonance (EPR), nuclear paramagnetic resonance (NMR) spectroscopy. - Dr. Grama László
• 13. High-resolution structural approaches 1. X-ray crystallography. - Dr. Talián Csaba Gábor
• 14. High-resolution structural approaches 2. SAXS, EM, cryo-EM. - Dr. Lukács András Szilárd
• 15. Imaging techniques. Basics of light microscopy. - Dr. Bugyi Beáta
• 16. Fluorescence microscopy. - Dr. Bugyi Beáta
• 17. Advanced fluorescence microscopy. - Dr. Bugyi Beáta
• 18. Image analysis. - Dr. Bugyi Beáta
• 19. Nuclear physics, radioactivity. - Dr. Szabó-Meleg Edina
• 20. Biological effects of radioactive radiations, dosimetry. - Dr. Szabó-Meleg Edina
• 21. Medical imaging techniques in diagnostics 1. Gamma-camera, computed tomography (CT), single-photon emission computed tomography (SPECT) - Dr. Grama László
• 22. Medical imaging techniques in diagnostics 2. Positron emission tomography (PET), magnetic resonance imaging (MRI) - Dr. Grama László
• 23. Thermodynamics, thermal analysis. - Dr. Lukács András Szilárd
• 24. Calorimetric methods: differential scanning calorimetry (DSC), isothermal titration calorimetry (ITC). - Dr. Lukács András Szilárd
• 25. Analytical separation techniques: sedimentation, electrophoresis, chromatography. - Dr. Bukovics Péter
• 26. Mass spectrometry. - Dr. Talián Csaba Gábor
• 27. Revision, Students' project presentations. - Dr. Bugyi Beáta
• 28. Revision, Students' project presentations. - Dr. Bugyi Beáta

Practices

Seminars

Reading material

Obligatory literature

Literature developed by the Department

Homepage of the Department of Biophysics:
https://aok.pte.hu/en/egyseg/10/oktatasi-anyagok

Notes

Recommended literature

Conditions for acceptance of the semester

Maximum of 15 % absence allowed

Mid-term exams

Theory exams are scheduled in the exam period according to the rules of studies and examinations.

Making up for missed classes

The opportunity to make up for absence is not provided.

Exam topics/questions

Topics of the exam questions:
Electromagnetic waves
Quantum numbers, NMR, MRI
Energy levels of atoms and molecules
Laser
UV-VIS absorption spectroscopy
Fluorescence spectroscopy
Infrared and Raman spectroscopy
Rapid kinetic methods
Flow cytometry
Light and fluorescence microscopy
Advanced fluorescence microscopy
Image analysis
Sound, ultrasound
Gamma camera, computed tomography (CT), single-photon emission computed tomography (SPECT), positron emission tomography (PET)
Radioactivity, the interaction of radioactive radiations with matter
Biological effects of radioactive radiations, dosimetry
X-ray diffraction
Thermodynamics: laws and thermodynamic potentials
Calorimetry: differential scanning calorimetry (DSC), isothermal titration calorimetry (ITC)
Analytical separation techniques: sedimentation, electrophoresis
Analytical separation techniques: chromatographic techniques
Mass spectrometry

Examiners

• Dr. Bugyi Beáta
• Dr. Grama László
• Dr. Huberné Dr. Barkó Szilvia
• Dr. Lukács András Szilárd
• Dr. Szabó-Meleg Edina
• Dr. Talián Csaba Gábor

Instructor / tutor of practices and seminars