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Biotechnological Methods in Forensic Medicine

Data

Official data in SubjectManager for the following academic year: 2024-2025

Course director

Number of hours/semester

lectures: 12 hours

practices: 12 hours

seminars: 0 hours

total of: 24 hours

Subject data

  • Code of subject: OBF-BMF-T
  • 2 kredit
  • Biotechnology MSc
  • Optional modul
  • autumn
Prerequisites:

-

Course headcount limitations

min. 5 – max. 9

Available as Campus course for . Campus-karok: TTK

Topic

These days the importance of laboratory and molecular biology methods is obvious in the field of forensic medicine. These methods are used in the examination of different body fluids (blood, saliva, vaginal secretum). During the course we will discuss in details the methods used to analyze DNA (identifications, paternity testing): the significance of analyses of STRs, SNPs, and mitochodrial DNA. Besides the basic DNA methods we talk about new scientific approaches which are based on RNA and protein analyses (determination of course and time of death).

Lectures

  • 1. Sample collection, DNA extraction, specialities in Forensic Science - Poór Viktor Soma
  • 2. Forensic histology - Tóth Dénes
  • 3. Biology of short tandem repeats - Poór Viktor Soma
  • 4. The Chelex method - Fejes Vivien
  • 5. Next generation DNA sequencing - Poór Viktor Soma
  • 6. Identification of body fluids - Poór Viktor Soma
  • 7. Kinship analysis - Poór Viktor Soma
  • 8. Polymerase chain reaction - Poór Viktor Soma
  • 9. Forensic autopsy - Tóth Dénes
  • 10. Forensic applications of next generation sequencing - Poór Viktor Soma
  • 11. Diagnosis of drowning - Jánosa-Szűcs Dominika
  • 12. Examination - Poór Viktor Soma

Practices

  • 1. DNA extraction - Poór Viktor Soma
  • 2. Forensic histology - Tóth Dénes
  • 3. Polymerase chain reaction - Poór Viktor Soma
  • 4. DNA extraction with Chelex - Fejes Vivien
  • 5. Analysis of genetic profiles - Poór Viktor Soma
  • 6. Analysis of genetic profiles - Poór Viktor Soma
  • 7. Identification of biological stains - Poór Viktor Soma
  • 8. Identification of biological stains - Poór Viktor Soma
  • 9. Forensic autopsy - Tóth Dénes
  • 10. Sequencing methods - Poór Viktor Soma
  • 11. Diatom testing - Fejes Vivien
  • 12. Consultation and exam - Poór Viktor Soma

Seminars

Reading material

Obligatory literature

Literature developed by the Department

Notes

Recommended literature

Alan Gunn: Essential Forensic Biology,

Forensic DNA Typing, John M. Butler; Molecular Forensics, Ralph Rapley;

DNA: Forensic and Legal Applications Kobilinsky L.

Conditions for acceptance of the semester

Participation in the seminars and practices.

Mid-term exams

Written exam on the last practice or presentation on a forensic medicine related topic

Making up for missed classes

Based on personal agreement

Exam topics/questions

Subjects of the exam are given out on the lectures

Examiners

Instructor / tutor of practices and seminars

  • Fejes Vivien
  • Jánosa-Szűcs Dominika
  • Poór Viktor Soma
  • Tóth Dénes
Rólunk