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Supervisor: HORVÁTH-SZALAI, Zoltán
Serum vitamin D binding protein (VDBP) is an α2-globulin (molecular weight: 52-59 kDa) produced by the liver. It has 3 main isoforms (Gc1, Gc1-2, Gc2), in addition, about 120 different variants have been described so far. The frequency of certain isoforms is higher in some diseases (chronic obstructive pulmonary disease, diabetes), however, the occurence of the isoforms in intensive care-related disorders has not been studied yet. VDBP is the major carrier of vitamin D in the blood: 99% of vitamin D metabolites (mostly 25(OH) vitamin D) are bound by this protein and transported to the tissues. According to the free hormone hypothesis, mostly free 25(OH) vitamin D can enter the cells and – after 1-α-hydroxilation – exert its physiologic effects. Currently, serum total 25(OH) vitamin D levels are considered the gold standard marker to evaluate overall vitamin D status, however, free 25(OH) vitamin D levels may offer additional information on the vitamin D status and on possible unfavourable disease outcomes. We aim to investigate serum VDBP isoforms by native polyacrilamide gel electrophoresis coupled with immunoblot technique, to measure total 25(OH) vitamin D levels, in addition, to calculate free 25(OH) vitamin D concentration in intensive care patients.