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Use of the EPR Spectroscopy on Biological Structures

Data

Official data in SubjectManager for the following academic year: 2024-2025

Course director

Number of hours/semester

lectures: 6 hours

practices: 6 hours

seminars: 0 hours

total of: 12 hours

Subject data

  • Code of subject: OXF-EPR-h-T
  • 1 kredit
  • General Medicine
  • Optional modul
  • both
Prerequisites:

ODA-BI1-T finished

Course headcount limitations

min. 5 – max. 6

Available as Campus course for . Campus-karok: ETK TTK

Topic

The aim of the course is to learn the usual techniques in EPR spectroscopy on different samples, and evaluation of the results obtained.

The electron paramagnetic resonance spectroscopy is a method similar to NMR, but deals with the properties of electron spin resonance. The technique provides data on the molecular structure and dynamics of various, e.g. biological systems.

The investigation of paramagnetic materials, like free radicals, metals, and special spin-labels help to describe biological systems, e.g. membranes, proteins.

Lectures

  • 1. The basics of EPR Spectroscopy - Borbásné Farkas Kornélia
  • 2. The basic features of EPR Spectrometer - Borbásné Farkas Kornélia
  • 3. Spin labels and labeling methods - Dergez Tímea
  • 4. Free radicals - spin trapping - Dergez Tímea
  • 5. Membrane structures and their labeling - Dergez Tímea
  • 6. Other biological structures and their labeling - Dergez Tímea

Practices

  • 1. Way of registration, registration and analyze of an EPR spectra - Dergez Tímea
  • 2. EPR spectra analysis of membrane dynamics 1. - Dergez Tímea
  • 3. EPR spectra analysis of membrane dynamics 2. - Dergez Tímea
  • 4. EPR spectra analysis of biological structures 1. (muscle fibres) - Dergez Tímea
  • 5. EPR spectra analysis of other biological structures 2. (with spin labelling technics) - Dergez Tímea
  • 6. Spin trapping method - Dergez Tímea

Seminars

Reading material

Obligatory literature

Literature developed by the Department

Notes in Microsoft Teams Group.

Notes

Recommended literature

1. C. P. Poole: Electron Spin Resonance, John Wiley & Sons, New York, 1983

2. Hoppe, N., Lohmann, W., Marke, H., Ziegler, H.: Biophysics, Springer-Verlag, Berlin, 1987

Conditions for acceptance of the semester

6 tasks to solve.

Mid-term exams

There are 6 homeworks (all with 5 short questions).

Making up for missed classes

Consultation

Exam topics/questions

List of the exam can be find in Microsoft Teams Group.

Examiners

Instructor / tutor of practices and seminars

  • Borbásné Farkas Kornélia
  • Dergez Tímea
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