Data
Official data in SubjectManager for the following academic year: 2024-2025
Course director
-
Dergez Tímea
assistant professor,
Institute of Bioanalysis -
Number of hours/semester
lectures: 6 hours
practices: 6 hours
seminars: 0 hours
total of: 12 hours
Subject data
- Code of subject: OXF-EPR-h-T
- 1 kredit
- General Medicine
- Optional modul
- both
ODA-BI1-T finished
Course headcount limitations
min. 5 – max. 6
Available as Campus course for . Campus-karok: ETK TTK
Topic
The aim of the course is to learn the usual techniques in EPR spectroscopy on different samples, and evaluation of the results obtained.
The electron paramagnetic resonance spectroscopy is a method similar to NMR, but deals with the properties of electron spin resonance. The technique provides data on the molecular structure and dynamics of various, e.g. biological systems.
The investigation of paramagnetic materials, like free radicals, metals, and special spin-labels help to describe biological systems, e.g. membranes, proteins.
Lectures
- 1. The basics of EPR Spectroscopy - Borbásné Farkas Kornélia
- 2. The basic features of EPR Spectrometer - Borbásné Farkas Kornélia
- 3. Spin labels and labeling methods - Dergez Tímea
- 4. Free radicals - spin trapping - Dergez Tímea
- 5. Membrane structures and their labeling - Dergez Tímea
- 6. Other biological structures and their labeling - Dergez Tímea
Practices
- 1. Way of registration, registration and analyze of an EPR spectra - Dergez Tímea
- 2. EPR spectra analysis of membrane dynamics 1. - Dergez Tímea
- 3. EPR spectra analysis of membrane dynamics 2. - Dergez Tímea
- 4. EPR spectra analysis of biological structures 1. (muscle fibres) - Dergez Tímea
- 5. EPR spectra analysis of other biological structures 2. (with spin labelling technics) - Dergez Tímea
- 6. Spin trapping method - Dergez Tímea
Seminars
Reading material
Obligatory literature
Literature developed by the Department
Notes in Microsoft Teams Group.
Notes
Recommended literature
1. C. P. Poole: Electron Spin Resonance, John Wiley & Sons, New York, 1983
2. Hoppe, N., Lohmann, W., Marke, H., Ziegler, H.: Biophysics, Springer-Verlag, Berlin, 1987
Conditions for acceptance of the semester
6 tasks to solve.
Mid-term exams
There are 6 homeworks (all with 5 short questions).
Making up for missed classes
Consultation
Exam topics/questions
List of the exam can be find in Microsoft Teams Group.
Examiners
Instructor / tutor of practices and seminars
- Borbásné Farkas Kornélia
- Dergez Tímea