Protein Bioanalytics - Lecture

Data

Official data in SubjectManager for the following academic year: 2024-2025

Course director

Number of hours/semester

lectures: 28 hours

practices: 0 hours

seminars: 0 hours

total of: 28 hours

Subject data

  • Code of subject: OBA-109-E
  • 2 kredit
  • Biotechnology MSc
  • Basic modul
  • spring
Prerequisites:

-

Course headcount limitations

min. 5

Topic

The purpose of this subject is to describe and characterize proteins, which are the most important components in the cell-function. Analysis of the proteome with molecular biology, bioinformatics and biostatistical methods. Structure-function relationships in proteins. Protein associates. Methodology to study the structure of proteins (NMR, mass spectrometry). Purification of proteins. Bioanalysis of proteins.

Lectures

  • 1. Basic description of protein structure - Fenyvesiné Páger Csilla
  • 2. Basic description of protein structure - Fenyvesiné Páger Csilla
  • 3. Domain-multidomain setups and functions - Fenyvesiné Páger Csilla
  • 4. Domain-multidomain setups and functions - Fenyvesiné Páger Csilla
  • 5. Structure investigation with advanced methods. NMR of proteins - Berente Zoltán
  • 6. Structure investigation with advanced methods. NMR of proteins - Berente Zoltán
  • 7. Mass spectrometry in proteomics - Kilár Anikó Gyöngyvér
  • 8. Mass spectrometry in proteomics - Kilár Anikó Gyöngyvér
  • 9. Protein networks - Nagy Laura
  • 10. Protein networks - Nagy Laura
  • 11. Crystallization of proteins. - Nagy Laura
  • 12. X-ray crystallography - Nagy Laura
  • 13. Posttranslational modifications I: Glycomics - Kilár Ferenc
  • 14. Posttranslational modifications I: Glycomics - Kilár Ferenc
  • 15. Posttranslational modifications II - Nagy Laura
  • 16. Posttranslational modifications II - Nagy Laura
  • 17. Protein modelling - Kilár Ferenc
  • 18. Protein modelling - Kilár Ferenc
  • 19. Protein purification methods - Fenyvesiné Páger Csilla
  • 20. Protein purification methods - Nagy Laura
  • 21. Protein-protein interactions - Nagy Laura
  • 22. Protein-protein interactions - Nagy Laura
  • 23. Protein databases - Nagy Laura
  • 24. Protein databases - Nagy Laura
  • 25. Surface interaction methods - Kilár Anikó Gyöngyvér
  • 26. Surface interaction methods - Kilár Anikó Gyöngyvér
  • 27. Signal transduction of proteins - Nagy Laura
  • 28. Signal transduction of proteins - Nagy Laura

Practices

Seminars

Reading material

Obligatory literature

Literature developed by the Department

Notes

Recommended literature

F. Lottspeich and J. W. Engels (eds.): Bioanalytics. Bioanalytical Methods and Concepts in Biochemistry and Molecular Biology, Wiley-VCH, Weinheim, Germany, 2018

D. Liu and F-Y. Wan (eds.): Advances in Computational Intelligence: Theory & Applications,,

World Scientific, Hackensack, 2006

Petsko, Gregory A./ Ringe, Dagmar: Protein Structure and Function, Sinauer Associates Inc., 2004

Buxbaum, Engelbert: Fundamentals of Protein Structure and Function, Springer, 2007

Conditions for acceptance of the semester

According to the Faculty rules

Mid-term exams

According to the Faculty rules

Making up for missed classes

Retake is not possible.

Exam topics/questions

Website of the Institute of Bioanalysis under institute of Bioanalysis/Education/Educational materials.

Examiners

  • Fenyvesiné Páger Csilla
  • Kilár Anikó Gyöngyvér
  • Kilár Ferenc
  • Nagy Laura

Instructor / tutor of practices and seminars