Data
Official data in SubjectManager for the following academic year: 2023-2024
Course director
-
Dr. Attila FELINGER
professor,
Institute of Bioanalysis -
Number of hours/semester
lectures: 28 hours
practices: 0 hours
seminars: 0 hours
total of: 28 hours
Subject data
- Code of subject: OBA-109-E
- 2 kredit
- Biotechnology MSc
- Basic modul
- spring
-
Exam course:yes
Course headcount limitations
min. 5
Topic
The purpose of this subject is to describe and characterize proteins, which are the most important components in the cell-function. Analysis of the proteome with molecular biology, bioinformatics and biostatistical methods. Structure-function relationships in proteins. Protein associates. Methodology to study the structure of proteins (NMR, mass spectrometry). Purification of proteins. Bioanalysis of proteins.
Lectures
- 1. Basic description of protein structure - Fenyvesiné Dr. Páger Csilla
- 2. Basic description of protein structure - Fenyvesiné Dr. Páger Csilla
- 3. Domain-multidomain setups and functions - Fenyvesiné Dr. Páger Csilla
- 4. Domain-multidomain setups and functions - Fenyvesiné Dr. Páger Csilla
- 5. Structure investigation with advanced methods. NMR of proteins - Dr. Berente Zoltán
- 6. Structure investigation with advanced methods. NMR of proteins - Dr. Berente Zoltán
- 7. Mass spectrometry in proteomics - Dr. Kilár Anikó Gyöngyvér
- 8. Mass spectrometry in proteomics - Dr. Kilár Anikó Gyöngyvér
- 9. Protein networks - Nagy Laura
- 10. Protein networks - Nagy Laura
- 11. Crystallization of proteins. - Nagy Laura
- 12. X-ray crystallography - Nagy Laura
- 13. Posttranslational modifications I: Glycomics - Dr. Kilár Ferenc
- 14. Posttranslational modifications I: Glycomics - Dr. Kilár Ferenc
- 15. Posttranslational modifications II - Nagy Laura
- 16. Posttranslational modifications II - Nagy Laura
- 17. Protein modelling - Dr. Kilár Ferenc
- 18. Protein modelling - Dr. Kilár Ferenc
- 19. Protein purification methods - Fenyvesiné Dr. Páger Csilla
- 20. Protein purification methods - Nagy Laura
- 21. Protein-protein interactions - Nagy Laura
- 22. Protein-protein interactions - Nagy Laura
- 23. Protein databases - Nagy Laura
- 24. Protein databases - Nagy Laura
- 25. Surface interaction methods - Dr. Kilár Anikó Gyöngyvér
- 26. Surface interaction methods - Dr. Kilár Anikó Gyöngyvér
- 27. Signal transduction of proteins - Nagy Laura
- 28. Signal transduction of proteins - Nagy Laura
Practices
Seminars
Reading material
Obligatory literature
Literature developed by the Department
Notes
Recommended literature
F. Lottspeich and J. W. Engels (eds.): Bioanalytics. Bioanalytical Methods and Concepts in Biochemistry and Molecular Biology, Wiley-VCH, Weinheim, Germany, 2018
D. Liu and F-Y. Wan (eds.): Advances in Computational Intelligence: Theory & Applications,,
World Scientific, Hackensack, 2006
Petsko, Gregory A./ Ringe, Dagmar: Protein Structure and Function, Sinauer Associates Inc., 2004
Buxbaum, Engelbert: Fundamentals of Protein Structure and Function, Springer, 2007
Conditions for acceptance of the semester
Maximum of 25 % absence allowed
Mid-term exams
Retake is not possible.
Making up for missed classes
According to the Faculty rules
Exam topics/questions
Website of the Institute of Bioanalysis under institute of Bioanalysis/Education/Educational materials.
Examiners
- Dr. Kilár Anikó Gyöngyvér
- Dr. Kilár Ferenc
- Fenyvesiné Dr. Páger Csilla
- Nagy Laura