Capillary Electrophoresis in Pharmaceutical Application


Official data in SubjectManager for the following academic year: 2023-2024

Course director

Number of hours/semester

lectures: 12 hours

practices: 0 hours

seminars: 0 hours

total of: 12 hours

Subject data

  • Code of subject: OXF-KFO-h-T
  • 1 kredit
  • General Medicine
  • Optional modul
  • autumn


Course headcount limitations

min. 5 – max. 50

Available as Campus course for 50 fő számára. Campus-karok: ETK TTK


The course presents six different methods of capillary electrophoresis, starting with the theoretical foundations and explaining various pharmaceutical applications. It provides an insight into the difficulties of the mass spectrometry connection and its development.
Capillary electrophoresis can be used as a modern analytical separation technique for the separation and determination of metal ions, inorganic anions, chiral compounds, peptides and proteins, carbohydrates, and even whole cells and viruses. It is also suitable for determining the physico-chemical characteristics of pharmaceutical compounds and testing their medicinal effectiveness.


  • 1. Evolution of separation methods - Fenyvesiné Dr. Páger Csilla
  • 2. Development of new electrophoretic methods - Fenyvesiné Dr. Páger Csilla
  • 3. Theory of electrophoresis - Fenyvesiné Dr. Páger Csilla
  • 4. Separation modes in electrophoresis, Zone electrophoresis - Fenyvesiné Dr. Páger Csilla
  • 5. Isoelectric focusing theory, IEF methodology, sandwich injection - Fenyvesiné Dr. Páger Csilla
  • 6. Isotachophoresis - Fenyvesiné Dr. Páger Csilla
  • 7. Gel electrophoresis - Fenyvesiné Dr. Páger Csilla
  • 8. High resolution analysis of macromolecules - Fenyvesiné Dr. Páger Csilla
  • 9. Chiral analysis of drugs - Fenyvesiné Dr. Páger Csilla
  • 10. Lab-on-a-chip methodology - Fenyvesiné Dr. Páger Csilla
  • 11. Application in laboratory diagnostics - Fenyvesiné Dr. Páger Csilla
  • 12. Coupling with mass spectrometry - Fenyvesiné Dr. Páger Csilla



Reading material

Obligatory literature

Literature developed by the Department


Recommended literature

James Landers (ed.): Handbook of Capillary Electrophoresis, CRC Press, 1994
Ann Van Schepdael: Microchip Capillary Electrophoresis Protocols, Springer, 2015

Conditions for acceptance of the semester

Maximum of 15 % absence allowed

Mid-term exams

Form of exam: Test (a score of 50% is required for a sufficient grade)

The required information for the test will be explained in the course.

Making up for missed classes

By personal consultation.

Exam topics/questions


Instructor / tutor of practices and seminars