Data
Official data in SubjectManager for the following academic year: 2024-2025
Course director
-
Bufa Anita
assistant professor,
Institute of Bioanalysis -
Number of hours/semester
lectures: 6 hours
practices: 6 hours
seminars: 0 hours
total of: 12 hours
Subject data
- Code of subject: OAF-VSP-T
- 1 kredit
- General Medicine
- Optional modul
- both
-
Course headcount limitations
min. 5 – max. 10
Available as Campus course for . Campus-karok: GYTK
Topic
The participants are going to be familiarised theoretically and practically with:
- pre-treatment of urine sample
- qualitative and quantitative analysis of 24 androgen, progesterone and corticoid metabolites by gas chromatography using mass selective detector
- evaluation of urinary steroid profiles
Lectures
- 1. Urinary steroid metabolites - Bufa Anita
- 2. Pre-treatment of urine sample - Bufa Anita
- 3. Gas chromatography-Mass spectrometry - Bufa Anita
- 4. Qualitative analysis of urinary steroid metabolites - Bufa Anita
- 5. Quantitative analysis of urinary steroid metabolites - Bufa Anita
- 6. Evaluation of urinary steroid profile - Bufa Anita
Practices
- 1. Solid-phase extraction of urinary steroid metabolites - Bufa Anita
- 2. Enzymatic hydrolysis of urinary steroid metabolites - Bufa Anita
- 3. Derivatization of urinary steroid metabolites - Bufa Anita
- 4. Chromatographic cleaning - Bufa Anita
- 5. Determination of urinary steroid profiles by GC-MS technic - Bufa Anita
- 6. Qualitative and quantitative analysis of urinary steroid metabolites - Bufa Anita
Seminars
Reading material
Obligatory literature
Literature developed by the Department
It is going to be available on the website of Teams group and PotePedia.
Notes
Recommended literature
Conditions for acceptance of the semester
After the course a written essay.
Mid-term exams
After the course a written essay.
Making up for missed classes
Consultation with course director is required. The necessary information is going to be presented on the course.
Exam topics/questions
The necessary information is going to be presented on the course.
Examiners
Instructor / tutor of practices and seminars
- Bufa Anita