Urinary Steroid Profiles

Data

Official data in SubjectManager for the following academic year: 2024-2025

Topic

The participants are going to be familiarised theoretically and practically with:

- pre-treatment of urine sample

- qualitative and quantitative analysis of 24 androgen, progesterone and corticoid metabolites by gas chromatography using mass selective detector

- evaluation of urinary steroid profiles

Lectures

• 1. Urinary steroid metabolites - Bufa Anita
• 2. Pre-treatment of urine sample - Bufa Anita
• 3. Gas chromatography-Mass spectrometry - Bufa Anita
• 4. Qualitative analysis of urinary steroid metabolites - Bufa Anita
• 5. Quantitative analysis of urinary steroid metabolites - Bufa Anita
• 6. Evaluation of urinary steroid profile - Bufa Anita

Practices

• 1. Solid-phase extraction of urinary steroid metabolites - Bufa Anita
• 2. Enzymatic hydrolysis of urinary steroid metabolites - Bufa Anita
• 3. Derivatization of urinary steroid metabolites - Bufa Anita
• 4. Chromatographic cleaning - Bufa Anita
• 5. Determination of urinary steroid profiles by GC-MS technic - Bufa Anita
• 6. Qualitative and quantitative analysis of urinary steroid metabolites - Bufa Anita

Seminars

Reading material

Obligatory literature

Literature developed by the Department

It is going to be available on the website of Teams group and PotePedia.

Notes

Recommended literature

Conditions for acceptance of the semester

After the course a written essay.

Mid-term exams

After the course a written essay.

Making up for missed classes

Consultation with course director is required. The necessary information is going to be presented on the course.

Exam topics/questions

The necessary information is going to be presented on the course.

Examiners

Instructor / tutor of practices and seminars

• Bufa Anita