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Capillary Electrophoresis in Pharmaceutical Application

Data

Official data in SubjectManager for the following academic year: 2024-2025

Course director

Number of hours/semester

lectures: 12 hours

practices: 0 hours

seminars: 0 hours

total of: 12 hours

Subject data

  • Code of subject: OPF-KFO-T
  • 1 kredit
  • Pharmacy
  • Optional modul
  • autumn
Prerequisites:

-

Course headcount limitations

min. 5 – max. 50

Available as Campus course for . Campus-karok: ETK TTK

Topic

The course presents six different methods of capillary electrophoresis, starting with the theoretical foundations and explaining various pharmaceutical applications. It provides an insight into the difficulties of the mass spectrometry connection and its development.

Capillary electrophoresis can be used as a modern analytical separation technique for the separation and determination of metal ions, inorganic anions, chiral compounds, peptides and proteins, carbohydrates, and even whole cells and viruses. It is also suitable for determining the physico-chemical characteristics of pharmaceutical compounds and testing their medicinal effectiveness.

Lectures

  • 1. Evolution of separation methods - Fenyvesiné Páger Csilla
  • 2. Development of new electrophoretic methods - Fenyvesiné Páger Csilla
  • 3. Theory of electrophoresis - Fenyvesiné Páger Csilla
  • 4. Separation modes in electrophoresis, Zone electrophoresis - Fenyvesiné Páger Csilla
  • 5. Isoelectric focusing theory, IEF methodology, sandwich injection - Fenyvesiné Páger Csilla
  • 6. Isotachophoresis - Fenyvesiné Páger Csilla
  • 7. Gel electrophoresis - Fenyvesiné Páger Csilla
  • 8. High resolution analysis of macromolecules - Fenyvesiné Páger Csilla
  • 9. Chiral analysis of drugs - Fenyvesiné Páger Csilla
  • 10. Lab-on-a-chip methodology - Fenyvesiné Páger Csilla
  • 11. Application in laboratory diagnostics - Fenyvesiné Páger Csilla
  • 12. Coupling with mass spectrometry - Fenyvesiné Páger Csilla

Practices

Seminars

Reading material

Obligatory literature

Literature developed by the Department

Notes

Recommended literature

James Landers (ed.): Handbook of Capillary Electrophoresis, CRC Press, 1994

Ann Van Schepdael: Microchip Capillary Electrophoresis Protocols, Springer, 2015

Conditions for acceptance of the semester

No additional conditions.

Mid-term exams

Form of exam: Test (a score of 50% is required for a sufficient grade)

The required information for the test will be explained in the course.

Making up for missed classes

By personal consultation.

Exam topics/questions

https://aok.pte.hu/hu/egyseg/160/oktatas

Examiners

Instructor / tutor of practices and seminars

Rólunk