Qualitative and Quantitative Analysis of Medicines and Psychoactive Substances from Biological Samples

Data

Official data in SubjectManager for the following academic year: 2024-2025

Topic

The course provides knowledge about the traceability of active pharmaceutical ingredients in various biological matrices. It discusses sampling of biological matrices, sample preparation (extraction) methods [LLE, SPE, SPEC, SPME, MEPS], and various chromatographic techniques [TLC, GC, LC] as well.

The course intends to present the CNS effects of active pharmaceutical ingredients, as well as drugs in the official investigation. The sampling procedures and techniques are presented, as well as their official confidence circumstances.

The appearance of drug compounds and drugs in biological matrices, and their pharmacokinetic properties are discussed, as well as traceability of live and corpse biological matrices (blood, urine, hair, nails, saliva, or liver, kidney, brain, or cerebrospinal fluid).

After proper selection of analytical tests, the requirements set for a variety of measuring instruments will be discussed. Standard evaluation and communication, as well as the laws and regulations in force in the analytical results complete the course topics.

Lectures

• 1. Introduction to Toxicology. Categorization of Toxins I - Csikós Eszter
• 2. Introduction to Toxicology. Categorization of Toxins II - Csikós Eszter
• 3. Effect of Psychoactive Substances on the Living Body I - Ács Kamilla
• 4. Effect of Psychoactive Substances on the Living Body II - Ács Kamilla
• 5. Investigation in Forensic Laboratories. Sampling rules. I - Horváth Adrienn (Farmakognózia)
• 6. Investigation in Forensic Laboratories. Sampling rules. II - Horváth Adrienn (Farmakognózia)
• 7. Investigation of Non-standard Human Tissues I - Balázs Viktória Lilla
• 8. Investigation of Non-standard Human Tissues II - Balázs Viktória Lilla
• 9. Biological Materials Preparation. Extraction Procedures I - Horváth Adrienn (Farmakognózia)
• 10. Biological Materials Preparation. Extraction Procedures II - Horváth Adrienn (Farmakognózia)
• 11. Forensic Toxicological Investigation of Human Samples by Chromatography I - Balázs Viktória Lilla
• 12. Forensic Toxicological Investigation of Human Samples by Chromatography II - Balázs Viktória Lilla
• 13. Criteria and Evaluation. Expert Opinion in Forensic Toxicology I - Horváth Adrienn (Farmakognózia)
• 14. Criteria and Evaluation. Expert Opinion in Forensic Toxicology II - Horváth Adrienn (Farmakognózia)
• 15. Natural and Synthetic Cannabinoids I - Horváth Adrienn (Farmakognózia)
• 16. Natural and Synthetic Cannabinoids II - Horváth Adrienn (Farmakognózia)
• 17. Challenges of adulteration and internet trade of medicines I - Ács Kamilla
• 18. Challenges of adulteration and internet trade of medicines II - Ács Kamilla
• 19. First Generation of Amphetamines (ecstasy, speed) - Balázs Viktória Lilla
• 20. Second generation of disco drugs (catinon and derivatives) - Balázs Viktória Lilla
• 21. Opiates (morphine, codeine, heroine) I - Csikós Eszter
• 22. Opiates (morphine, codeine, heroine) II - Csikós Eszter
• 23. Cocaine - Balázs Viktória Lilla
• 24. Body-pack Syndrome. - Balázs Viktória Lilla
• 25. Designer drugs I - Csikós Eszter
• 26. Designer drugs II - Csikós Eszter
• 27. Final Student ppt Presentation - Ács Kamilla
• 28. Final test - Ács Kamilla

Practices

Seminars

Reading material

Obligatory literature

R.C. Baselt: Disposition of Toxic Drugs and Chemicals in Man, 6th ed., Biomedical Publications, Foster City, CA, 2002

A.C. Moffat, M.D. Osselton, B. Widdop: Clarke's Analysis of Drugs and Poisons, Pharmaceutical Press, London, 2004.

Literature developed by the Department

The Lecture presentations (ppt files) will be available from the website of the Department of Pharmacognosy.

Notes

Recommended literature

R.J. Flanagan, A. Taylor, I.D. Watson, R. Whelpton: Fundamentals of Analytical Toxicology, John Wiley & Sons, Hoboken, NJ, 2007.

Conditions for acceptance of the semester

preparation of a one-page thesis to be submitted

Mid-term exams

Students have to prove their knowledge of the course material by presenting a selected topic in a short (10 min) talk. The topic should be related to one of the topics of the course, and students should rely on their knowledge acquired in previous classes.

The results of the final test should be above 60%.

Making up for missed classes

There is no possibility to make up for the classes.

Exam topics/questions

Same as lecture topics.

Examiners

Instructor / tutor of practices and seminars

• Benkő András Antal