Biochemistry II

Data

Official data in SubjectManager for the following academic year: 2024-2025

Course director

Number of hours/semester

lectures: 14 hours

practices: 28 hours

seminars: 14 hours

total of: 56 hours

Subject data

  • Code of subject: OTV-BIC2-T
  • 4 kredit
  • Biotechnology BSc
  • Specialised Core Module modul
  • autumn
Prerequisites:

OTV-BIC1-T finished

Exam course:

Course headcount limitations

min. 1 – max. 100

Topic

Biochemistry II is built on the already known energy providing and biosynthetic pathways. We will study the metabolic regulations at different levels: cellular, tissues and whole organism. This means the most important information about enzymes and hormones: their synthesis and mechanisms of action. Especially enzymes are the subject of biotechnology: they are products as well as tools. Considering the human body, we discuss the energy supplies in fasting, sport, work, and also the most frequent pathobiochemical situations (diabetes mellitus). Special subject is the transmembrane processes, which are the targets of a large number of drugs.

Lectures

  • 1. Biomembranes - Jánosa Gergely
  • 2. Transport processes - Sipos Katalin
  • 3.

    Hormones

    - Farkas Viktória
  • 4. Amino acids: synthesis and degradation - Farkas Viktória
  • 5. Urea cycle - Sipos Katalin
  • 6. Purine and pyrimidine synthesis - Farkas Viktória
  • 7. Degradation of nucleotides - Farkas Viktória
  • 8. Neurotransmitters - Pap Ramóna
  • 9. Role of the liver in metabolism - Sipos Katalin
  • 10. Development of drugs - Pandur Edina
  • 11. Drugs in biotechnology - Pandur Edina
  • 12. Metabolism of drugs - Sipos Katalin
  • 13. Genetic diseases of metabolism - Pap Ramóna
  • 14. Consultation - Sipos Katalin

Practices

  • 1.

    General laboratory instructions. Making report of the practice.

    - Pap Ramóna
  • 2.

    General laboratory instructions. Making report of the practice.

    - Pap Ramóna
  • 3. Preparation of cell lysate. Acrylamide gel. - Jánosa Gergely
  • 4. Preparation of cell lysate. Acrylamide gel. - Jánosa Gergely
  • 5. Western blotting - Pap Ramóna
  • 6. Western blotting - Pap Ramóna
  • 7. Anorganic phosphate usage in fermentation - Farkas Viktória
  • 8. Anorganic phosphate usage in fermentation - Farkas Viktória
  • 9. Lactate dehydrogenase enzyme - Pap Ramóna
  • 10. Lactate dehydrogenase enzyme - Pap Ramóna
  • 11. Alkalic phosphatase. Inhibition of the enzyme - Pap Ramóna
  • 12. Alkalic phosphatase. Inhibition of the enzyme - Pap Ramóna
  • 13. Determination of blood sugar - Pap Ramóna
  • 14. Determination of blood sugar - Pap Ramóna
  • 15. Determination of cholesterol level - Farkas Viktória
  • 16. Determination of cholesterol level - Farkas Viktória
  • 17. Determination of uric acid level - Farkas Viktória
  • 18. Determination of uric acid level - Farkas Viktória
  • 19. HPLC - Horváth Adrienn (Farmakognózia)
  • 20. HPLC - Horváth Adrienn (Farmakognózia)
  • 21. Mass spectrometry - Horváth Adrienn (Farmakognózia)
  • 22. Mass spectrometry - Horváth Adrienn (Farmakognózia)
  • 23. Toxicology - determination of ethanol level in blood - Sipos Katalin
  • 24. Toxicology - determination of ethanol level in blood - Sipos Katalin
  • 25. Institute of Laboratory Medicine - human samples for diagnosis, automated systems in a laboratory - Sipos Katalin
  • 26. Institute of Laboratory Medicine - human samples for diagnosis, automated systems in a laboratory - Sipos Katalin
  • 27. Consultation - Sipos Katalin
  • 28. Consultation - Sipos Katalin

Seminars

  • 1. Biochemistry of vitamins - Jánosa Gergely
  • 2. Digestion and absorption of macronutrients - Pap Ramóna
  • 3.

    Transport processes

    - Sipos Katalin
  • 4. Diabetes mellitus - Farkas Viktória
  • 5. Muscle, brain, kidney, sport, pregnancy - Jánosa Gergely
  • 6. Starvation, fed state - Farkas Viktória
  • 7. Metabolism of lipids - Sipos Katalin
  • 8. Carbohydrate metabolism - Sipos Katalin
  • 9. Regulation of glycolysis, gluconeogenesis - Farkas Viktória
  • 10. Regulation of citric acid cycle, ATP synthesis - Sipos Katalin
  • 11. Regulation of glycogen and cholesterol metabolism - Farkas Viktória
  • 12. Metabolism of alcohol - Pap Ramóna
  • 13. Consultation - Farkas Viktória
  • 14. Consultation - Sipos Katalin

Reading material

Obligatory literature

Literature developed by the Department

The materials of the lectures and seminars will appear on Neptune.

Notes

Recommended literature

Ch. P. Woodbury: Biochemistry for the Pharmaceutical Sciences

Raymond S. Ochs: Biochemistry

Thomas M. Devlin: Textbook of Biochemistry with Clinical Correlations

Conditions for acceptance of the semester

There are no other terms of acceptance.

Mid-term exams

There is no written mid-term exam in the semester.

Making up for missed classes

According to personal agreement

Exam topics/questions

There are no given exam questions. The topics of the writtenexam will be the materials of lectures and seminars. There essay questions in the final exam about known topics.

Examiners

  • Farkas Viktória
  • Pandur Edina
  • Sipos Katalin

Instructor / tutor of practices and seminars

  • Farkas Viktória
  • Jánosa Gergely
  • Pap Ramóna
  • Sipos Katalin