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Biochemistry - Practice

Data

Official data in SubjectManager for the following academic year: 2024-2025

Course director

Number of hours/semester

lectures: 0 hours

practices: 28 hours

seminars: 0 hours

total of: 28 hours

Subject data

  • Code of subject: OBA-108-G
  • 2 kredit
  • Biotechnology MSc
  • Basic modul
  • autumn
Prerequisites:

OBA-108-E parallel

Course headcount limitations

min. 5 – max. 20

Topic

The course helps to evolve good practical skills. It gives an overview on the techniques that are used in the basic biochemical-, biomedical research, both in the industrial area as well as in clinical research. The main focus is on solving of different scientific problems and through that a solution orientated education.

Lectures

Practices

  • 1. Solutions - Takátsy Anikó
  • 2. Solutions - Takátsy Anikó
  • 3. Preparation of buffer solution - Takátsy Anikó
  • 4. Preparation of buffer solution - Takátsy Anikó
  • 5. Protein function - Takátsy Anikó
  • 6. Basics of enzyme catalysis - Takátsy Anikó
  • 7. Measuring the proteint contant of different solutions - Nagyné Kiss Gyöngyi
  • 8. Biuret reaction - Nagyné Kiss Gyöngyi
  • 9. Oral presentations - Takátsy Anikó
  • 10. Oral presentations - Takátsy Anikó
  • 11. Enzyme activity measurement - Takátsy Anikó
  • 12. Inorganic phosphate demand of glycolysis - Takátsy Anikó
  • 13. Oral presentations - Takátsy Anikó
  • 14. Oral presentations - Takátsy Anikó
  • 15. Molecular recognition - Nagyné Kiss Gyöngyi
  • 16. Affinity chromatography - Nagyné Kiss Gyöngyi
  • 17. Apoptosis, necrosis - Nagyné Kiss Gyöngyi
  • 18. Techniques to describe apoptotical processes or necrosis - Nagyné Kiss Gyöngyi
  • 19. PAGE, Western blot - Nagyné Kiss Gyöngyi
  • 20. PAGE, Western blot - Nagyné Kiss Gyöngyi
  • 21. Antibody-Based techniques in biochemistry - Juhász Kata
  • 22. Antibody-Based techniques in biochemistry - Juhász Kata
  • 23. Working with cells - Juhász Kata
  • 24. Working with cells - Juhász Kata
  • 25. Bioanalytical teachniques - Takátsy Anikó
  • 26. Bioanalytical teachniques - Takátsy Anikó
  • 27. Consultation - Takátsy Anikó
  • 28. Exam - Takátsy Anikó

Seminars

Reading material

Obligatory literature

Albert L. Lehninger - David L. Nelson - Michael M.Cox: Principles of Biochemistry, Worth Publichers, Inc.

Literature developed by the Department

Teams: OBA-108-G-2023241-MEA-A108-G-B1

http://aok.pte.hu/hu/egyseg/oktatasianyagok/20

Notes

Teams: OBA-108-G-2023241-MEA-A108-G-B1

http://aok.pte.hu/hu/egyseg/oktatasianyagok/20

Recommended literature

Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr., Lubert Stryer: Biochemistry

Thomas M. Devlin: Textbook of Biochemistry. With Clinical Correlations, John Wiley & Sons, New York

Conditions for acceptance of the semester

A written exam on the last week of the semester, or in the first two weeks of the exam periode

Mid-term exams

Oral presentations should be kept by every student on the 5th or 7th week from an optional topic, in the from of a ppt presentation. The theme of the presentation should have a biochemical connection, and should be adjusted with the subject leader.

A written exam is going to be written in the 14th week of the semester that screens the basic methodological knowledge, and the capability of solving scientific problems. In case of unsuccesful exam, it can be retaken in the first two weeks of the exam periode.

Making up for missed classes

There is no possibility to retake the practice.

Exam topics/questions

Teams: OBA-108-G-2023241-MEA-A108-G-B1

Examiners

Instructor / tutor of practices and seminars

  • Juhász Kata
  • Takátsy Anikó
Rólunk