In the Stimulated Emission Depletion (STED) microscope, the illumination is supplemented by a so-called STED laser beam. This STED beam is concentric with the excitation beam and has a donut-like intensity. The STED beam resets the excited fluorophores by forced emission, depending on its intensity, thus reducing the spot size where effective excitation occurs. With this method, the resolution in the x-y direction can be reduced to around 40 nm.
- Sytem: Abberiror Intruments Expert Line / FLIM,
- Frame: Nikon Eclipse Ti2
- Lasers: 488 nm, 561 nm, 640 nm
- STED lézer: 775 nm
- Objectives: Plan Flour 20x, SR HP Apo F 100xAC OIL
- Detektor: HybridGasp, APD